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相对超分辨率显微镜与深度紫外线反应激活

Kirti Prakash^1,2

¹Division of Molecular Pathology, The Institute of Cancer Research, London, UK.

Journal of microscopy

|December 25, 2023

概括

相对超分辨率显微镜结合了STED和SMLM等技术. 从水星弧灯中使用深紫外线波长可以恢复STED光漂白,并为SMLM成像重新激活光体.

科学领域:

光学显微镜是一种光学显微镜.
生物物理学的生物物理.
细胞生物学细胞生物学

背景情况:

相对超分辨率显微镜的目标是可视化超出衍射极限的生物结构.
结合确定性刺激发射耗尽 (STED) 和随机单分子定位显微镜 (SMLM) 提出了重大挑战.
关键的挑战包括STED期间的光白化和SMLM随后的光白复激活的需要.

研究的目的:

研究一种方法,以克服相对应的STED和SMLM中的光漂白限制.
为了证明深紫外线 (DBUE) 波长对光体再激活的实用性.
为了确定最佳的光体和成像介质,用于结合STED和SMLM.

主要方法:

利用来自水星 (Hg) 弧光灯的深紫外线 (DBUE) 波长.
应用DBUE辐射来恢复STED诱导的光漂白.
对随后的SMLM成像进行评估的光体活性.

主要成果:

深紫外线 (DBUE) 波长有效地恢复了STED诱导的光漂白.
DBUE辐射成功地为SMLM成像重新激活了单个分子.
亚历克萨594和Prolong Diamond被确定为相对应的STED和SMLM的合适光剂和成像介质.

结论:

深紫外线 (DBUE) 激发为相对应的STED和SMLM中的光体活性化提供了可行的解决方案.
这种方法促进了STED和SMLM模式的无整合.
亚历克萨594和Prolong Diamond的组合支持强大的相关超分辨率成像.

关键词:

在LFSM中,LFSM是LFSM.SMLMLM SMLMLM 这是一个很大的问题.子一个子紫外线的反激活方式相关显微镜的相关显微镜.单个分子成像技术超高分辨率的显微镜.

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