动态近距离相互作用分析表明,YPEL2参与了细胞应激监测
Gizem Turan1, Çağla Ece Olgun1, Hazal Ayten1
1Department of Biological Sciences, Middle East Technical University, Ankara, Türkiye.
Protein science : a publication of the Protein Society
|December 25, 2023
概括
YPEL2蛋白与RNA处理因子相互作用并局部化到压力颗粒,这表明它在细胞压力监测机制和RNA代谢中发挥作用.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 包括YPEL2在内的YPEL家族在进化过程中得到了保护,并参与了诸如增殖,分化和死亡等关键细胞过程.
- 基于YPEL2功能的精确分子机制在很大程度上仍未被描述.
- 了解蛋白质相互作用是解读细胞功能的关键.
研究的目的:
- 通过依赖近距离的生物化和质谱测量来确定YPEL2的交互伙伴.
- 根据其交互网络推断YPEL2的功能角色.
- 为了研究YPEL2在细胞应激反应中的参与.
主要方法:
- 动态TurboID合质谱法用于识别YPEL2近距离反应堆.
- 在COS7细胞中利用了诱导性转基因表达系统.
- 使用免疫光显微镜观察YPEL2在压力颗粒中的定位.
主要成果:
- YPEL2近距离相互作用体主要参与RNA和mRNA代谢,核糖核蛋白复合体生物发生和miRNA介导的基因沉默.
- 发现YPEL2与RNA结合蛋白ELAVL1和自受体SQSTM1.1相互作用.
- 观察到YPEL2在暴露于氧化应激诱导剂酸时局部化为应激颗粒.
结论:
- YPEL2的交互网络表明它在RNA处理和细胞应激反应中起着重要作用.
- YPEL2对应力颗粒的局部化表明它参与了应力颗粒动态.
- 这些发现确定YPEL2是细胞应激监测机制的潜在参与者.
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