在氨氧化过程中Fe和Cu可用性对同位素分离的生理影响
Donald E Martocello1,2, Scott D Wankel1
1Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, United States.
Environmental science & technology
|December 26, 2023
概括
金属的可用性通过影响氨氧化,影响循环. 这项研究揭示了铜和铁如何影响氨氧化细菌和古生物中的同位素分离,将环境因素与微生物生理学联系起来.
科学领域:
- 环境微生物学 环境微生物学
- 生物地质化学生物地质化学
- 同位素地球化学 同位素地球化学
背景情况:
- 微生物循环对于生态系统健康和全球生物地化学过程至关重要.
- 氨氧化是关键的化步骤,由氨氧化细菌 (AOB) 和古生物 (AOA) 进行.
- 这些微生物需要像铜 (Cu) 和铁 (Fe) 这样的微量金属来进行生长和酶功能.
研究的目的:
- 为了研究铁 (Fe) 和铜 (Cu) 在氨氧化过程中对同位素分离的影响.
- 为了确定金属的可用性是否通过影响微生物生长率或金属酶活性来调节动能同位素效应 (15εAO).
主要方法:
- 在AOB和AOA *Nitrosopumilus maritimus* SCM1.1.培养物中的Fe和Cu度的实验操纵.
- 在不同的金属可用性和生长条件下,测量氨氧化 (15εAO) 中动态同位素效应.
- 对氨单氧酶 (AMO) 活性增长速度和潜在调节的分析.
主要成果:
- 氨氧化细菌 (AOB) 显示15εAO与生长速度的一般独立性,除了在低温 (10°C) 的情况下.
- 氨氧化古生物 (*Nitrosopumilus maritimus* SCM1) 在较低的氧化率下表现出非线性减少15的AO.
- 这些观察到的动态表明生理上的约束影响异位平衡,以响应金属的可用性.
结论:
- 金属的可用性可以显著影响氨氧化过程中的同位素分离,AOB和AOA的反应不同.
- 该研究强调了环境因素 (金属可用性,温度) 和微生物生理学在控制循环过程中的相互作用.
- 调查结果为解释稳定同位素数据的循环和了解微生物适应环境条件提供了洞察力.
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