在MALDI-MS成像中用于组织内化学衍生的动员电喷装置
Andrew E Paulson1, Evan A Larson1, Young Jin Lee1
1Department of Chemistry, Iowa State University, Ames, Iowa 50011, United States.
Journal of the American Society for Mass Spectrometry
|December 26, 2023
概括
一种新型移动电喷装置增强了对大组织的矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI) 样本准备. 这项创新改进了组织内化学衍生物化 (OTCD) 和矩阵应用,从而实现了详细的组织分析.
科学领域:
- 分析化学 分析化学
- 质谱仪成像成像 质谱仪成像
- 生物化学 生物化学
背景情况:
- 矩阵辅助激光脱/离子化质谱成像 (MALDI-MSI) 需要均的矩阵或衍生剂应用.
- 目前使用雾化喷雾器的方法是移动的,而电子喷雾器通常是不移动的,限制了它们对大组织样本的使用.
- 组织上化学衍生物化 (OTCD) 对于分析复杂生物样本中的特定分析物,如脂肪化物至关重要.
研究的目的:
- 开发一种可移动的电喷装置,用于在MALDI-MSI中高效地准备大型组织样本.
- 评估设备在组织内化学衍生物化 (OTCD) 和矩阵应用中的性能.
- 为了研究静电电荷对OTCD过程中的反应加速的影响.
主要方法:
- 对商用自动雾化器设备的修改,以创建可移动的电喷喷雾器.
- 在模拟组织模型上使用吉拉德试剂T进行组织内化学衍生物化 (OTCD) 的演示.
- 静电充电与中性喷雾用于OTCD反应加速的比较.
- 该装置在老鼠大脑组织中用于MALDI-MSI脂肪化物,包括OTCD和矩阵应用.
主要成果:
- 开发的移动电喷装置成功为MALDI-MSI准备了大型组织样本.
- 该设备促进了高效的组织化学衍生物化 (OTCD) 和矩阵应用.
- 静电充电喷雾显示了加速OTCD反应的潜力.
- 通过使用该设备,在老鼠大脑组织中成功地实现了脂肪化物的MALDI-MSI.
结论:
- 移动电喷装置为大规模MALDI-MSI样本准备提供了多功能和有效的解决方案.
- 这项技术提高了组织内化学衍生物化 (OTCD) 的效率,并使具有挑战性的样本能够进行详细的分子成像.
- 这些发现为使用先进的质谱成像技术分析复杂生物组织中的生物分子开辟了新的途径.
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