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双向细胞外电子转移途径的Geobacter sulfurreducens生物膜:分子洞察细胞外聚合物质的分子洞察力

Guiqin Yang¹, Xue Xia¹, Weijie Nie¹

¹Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China.

Environmental research

|December 26, 2023

概括

电活性细菌 (EAB) 使用细胞外聚合物质 (EPS) 进行双向电子转移. 这项研究揭示了 Geobacter 生物膜中外向和内向电子转移之间的 EPS 组成和氧化还原活性差异.

科学领域:

生物电化学技术生物电化学技术
微生物的电化学
生物膜工程是生物膜的工程.

背景情况:

电活性细菌 (EAB) 通过电极促进双向细胞外电子转移 (EET).
细胞外聚合物物质 (EPS) 对于 EET 在 EAB 生物膜中至关重要.
在执行EET的Geobacter生物膜中对EPS的有限的生化和分子理解.

研究的目的:

探索Geobacter sulfurreducens生物膜中的双向EET机制.
为了描述EPS的组成和氧化还原特性.
阐明EPS在输出和输入EET中的作用.

主要方法:

构建 Geobacter sulfurreducens 的生物膜.
在EPS的电化学和光谱指纹.
对EPS的蛋白质组分析.

主要成果:

内向EET需要细胞外的氧化还原蛋白,其形式潜力低于外向EET.
阴极EPS (C-EPS) 的氧化还原活性低于阳极EPS (A-EPS),这是由于蛋白质/多糖合物比率和α-螺旋体含量发生变化所致.
在C-EPS中,细胞染色体较少,氨酸/氨酸类物质较多,这表明可用于内向EET的替代氧化还原介质.

结论:

在外向和内向EET之间,EPS组成和氧化还原活性显著不同.
细胞染色体在内向-EET中起到较小的作用,其他氧化还原蛋白可能参与其中.
在EPS中识别的氧化还原蛋白形成了一个支持双向EET的网络,这对生物电化学技术的进步至关重要.

关键词:

双向的细胞外电子转移.生物电化学系统生物电化学系统细胞外聚合物质是细胞外的聚合物质.生物薄膜中的Geobacter sulfurreducens是一种减少硫的生物薄膜.红氧活性蛋白是一种活性蛋白.

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