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基于LAMP和核酸合金技术的Fritillaria ussuriensis视觉识别的实验研究

Yu-He Ma¹, Yue Liu¹, Tao Li¹

¹School of Medical Technology, Beihua University, Jilin, 132013, China.

Analytical biochemistry

|December 26, 2023

概括

鉴定Fritillaria ussuriensis Maxim,一种传统的中国草药的真实性是一项挑战. 这项研究开发了一种使用循环介导异热放大 (LAMP) 和核酸合金的视觉识别方法,实现高特异性和灵敏度的可靠检测.

科学领域:

药理学是指药理学,即药理学是指药理学.
分子生物学分子生物学
分析化学分析化学

背景情况:

Fritillaria ussuriensis Maxim是一种有价值的中国传统草药.
准确识别F.ussuriensis至关重要,但在技术上具有挑战性.
现有的方法可能不足以提供可靠的身份验证.

研究的目的:

开发和验证用于识别Fritillaria ussuriensis Maxim.的快速,特定和视觉方法.
为了区分真正的F. ussuriensis与其伪造物和伪造产品.
为了评估商业上可用的F. ussuriensis样品的质量.

主要方法:

基于比较序列分析,为F. ussuriensis设计了特定的原料.
优化循环介导的同热放大 (LAMP) 用于视觉检测.
使用核酸合金测定方法验证了结果.
测试了20个商用样本.

主要成果:

开发了一种高度精确的 (100%) LAMP测定法用于F. ussuriensis的识别.
使用LAMP (0.01 ng μL−1) 和合金 (0.1 ng μL−1) 达到高灵敏度.
在20个商业样本中,确定了14个合格样本,其中6个不合格.

结论:

联合LAMP和合金方法为F. ussuriensis认证提供了一种特定,快速,精确和视觉的方法.
这种方法为中国传统草药的质量控制提供了新的技术解决方案.
这些发现凸显了市场上改的普遍性,以及需要可靠的识别技术的需求.

关键词:

F. 乌斯苏里南斯乌斯苏里南斯标识识别识别识别一个灯的灯光.核酸合金技术核酸合金技术视觉化的可视化

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