优化Coprinus comatus的液体发酵条件,以增强抗氧化活性
Dongyu Gu1,2, Shanshan Tang3, Chang Liu3
1School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian, China.
Preparative biochemistry & biotechnology
|December 26, 2023
概括
科普林斯康马图斯的发酵增强了它的抗氧化特性. 优化的发酵条件显著改善了2.2'-Azino-bis(3-ethylbenzothiazoline) -6-硫酸自由基的清除.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 科普林斯·科马图斯 (Coprinus comatus) 是因其食用和药用价值而闻名的植物.
- 研究其发酵产品可以揭示增强的生物活性特性.
- 抗氧化活性是药用真菌的一个关键属性.
研究的目的:
- 为了优化Coprinus comatus的发酵过程.
- 为了增强C. comatus发酵产品的抗氧化能力.
- 评估发酵提取物的自由基清除能力.
主要方法:
- 使用单因子和响应表面方法,优化了C. comatus的发酵.
- 通过测量2.2 -Azino-bis(3-ethylbenzothiazoline) -6-sulphonic acid (ABTS) 自由基的清除,评估了抗氧化活性.
- 确定n-butanol提取物的EC50值,以量化抗氧化剂的有效性.
主要成果:
- 科马图斯菌的发酵产品显示出显著的ABTS自由基清除能力.
- 优化的发酵条件 (14%C. comatus,C/N比率48:1,6天) 产生了最强的抗氧化活性.
- 在最佳条件下,n-butanol提取物的EC50为0.57±0.01 mg/mL,改善了12.31%.
结论:
- 发酵过程显著增强了Coprinus comatus的抗氧化潜力.
- 优化发酵条件对于最大限度地提高自由基清除活动至关重要.
- 这项研究为开发C. comatus作为抗氧化剂来源提供了基础.
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