相关实验视频

Updated: Jul 7, 2025

Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint

Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint

Published on: August 2, 2022

[BLOC1S1促进了山羊精子干细胞的增殖]

Shicheng Wan¹, Mengfei Zhang¹, Wenbo Chen¹

¹Shaanxi Stem Cell Engineering and Technology Research Center, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

|December 26, 2023

概括

溶酶体相关有机体复合体1亚单元1 (BLOC1S1) 的生物发生增强了山羊精子干细胞 (SSC) 的增殖. 这项研究创建了一个BLOC1S1-过度表达的SSC线,为抗病繁殖和理解精子生成提供了基础.

科学领域:

生殖生物学生殖生物学
干细胞科学干细胞科学
分子遗传学分子遗传学

背景情况:

精子干细胞 (SSC) 对于理解精子生成,动物育种和生殖疗法至关重要.
lysosome相关有机体复合体1亚单元1 (BLOC1S1) 的基因生物发生显示了对疾病耐药性的潜力.
研究BLOC1S1在山羊SSC中的作用可以推进抗病育种和生殖研究.

研究的目的:

要构建一个过度表达BLOC1S1.1.的山羊精子干细胞 (SSC) 线.
评估BLOC1S1过度表达对山羊SSC增殖的影响.
探索潜在的分子机制,包括EIF2S3Y/ERK通路.

主要方法:

使用同源重组构建了一个BLOC1S1过度表达向量.
通过lentivirus转染和puromycin选择生成了一个BLOC1S1-过度表达的山羊SSC线.
使用RT-qPCR量化过度表达;通过细胞生长曲线,流细胞计和EDU染色来评估增殖.

主要成果:

在工程性山羊SSC中实现了BLOC1S1表达的18倍增加.
在表达BLOC1S1过度表达的山羊SSC中显著增强了扩散活动.
观察到与增殖相关的基因 (PCNA,CDK2,CCND1) 和EIF2S3Y的上调,这表明EIF2S3Y/ERK通路的参与.

结论:

成功建立了一条山羊SSC线,由于BLOC1S1过度表达,其繁殖显著增强.
BLOC1S1促进了山羊的SSC增殖,可能通过EIF2S3Y/ERK通路.
这项研究提供了一个有价值的细胞平台,用于研究BLOC1S1的功能,并开发BLOC1S1过度表达的山羊用于繁殖.

关键词:

溶酶体相关细胞器官复合体1亚单元1 (BLOC1S1) 的生物发生一只山羊,一只山羊.它们的扩散和扩散.精子细胞的干细胞.

更多相关视频

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines

Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines

Published on: February 25, 2013

相关概念视频

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

Multipotency and Niche of Bulge Stem Cell

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

Cloning of Dolly the Sheep

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...