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Marie N Rapin¹, Lorna Murray², Ian H Sadler²

¹The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Daniel Rutherford Building, The King's Buildings, Edinburgh, UK.

Physiologia plantarum

|December 26, 2023

概括

沙罗菲特藻类拥有独特的阳离子多糖,称为"pectins",它们与陆地植物的pectins有很大不同. 这些Chlorokybus atmophyticus'pectins'显示出不同的糖成分和结构,表明植物细胞壁的复杂进化路径.

科学领域:

植物细胞壁生物学植物细胞壁生物学
藻类生物化学藻类生物化学
进化植物学进化植物学

背景情况:

陆地植物的细胞壁含有纤维素,半纤维素和阳离子pectin.
沙罗菲特藻类,陆地植物的亲属,共享一些细胞壁相似之处,但表现出主要的差异.
沙罗菲特"pectins"是可提取的,但在组成上与陆地植物的pectins不同.

研究的目的:

为了表征早期分离的炭植物Chlorokybus atmophyticus的阳离子多糖化物 ("pectins").
为了比较Chlorokybus'pectins'与陆地植物和其他链状植物的'pectins'.
阐明自从夏洛菲特和陆地植物分离以来,点结构的进化变化.

主要方法:

酸性水解以确定糖的组成 (GlcA,GalA,Ara,Glc,Gal,Xyl) 并确定特定的联系.
阴离子交换色谱以根据电荷来分离"pectin"部分.
酶性水解使用各种内分和外分水解来探测结构特征并确定联系.

主要成果:

基的"pectin"是阳离子的,产生了GlcA,GalA和硫酸盐,Ara和Glc是主要的中性糖;Rha不存在.
大多数银河糖是l-enantiomer,并确定了一个独特的β-d-GlcA-(1→4) -l-Gal脱糖化物.
用特定的水溶酶和"Driselase"进行的酶处理未能释放糖,这表明与陆地植物相比,这种植物具有不寻常的联系和结构.

结论:

克洛洛基布斯 (Chlorokybus atmophyticus) 含有阳性"性"多糖,可以发挥性作用,但在结构上与陆地植物素不同.
陆地植物pectin的进化涉及显著的变化,包括硫酸盐和l-Gal的损失,糖的重新配置和Rha.的增加.
这项研究突出了早期植物系中细胞壁组件的复杂和分歧的进化轨迹.

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