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甜桃AP2/ERF转录因子PavRAV2通过直接抑制PavKLUH表达来负面调节水果大小

Xiliang Qi^1,2, Lifeng Liu^1,2, Congli Liu^1,2

¹Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.

Physiologia plantarum

|December 26, 2023

概括

研究人员确定了一种关键基因,PavRAV2,通过控制PavKLUH的表达来调节甜桃水果的大小. 这一发现为植物育种更大的水果提供了洞察力.

科学领域:

植物生物学植物生物学
遗传学是一个遗传学.
农业科学农业科学

背景情况:

由于市场需求,水果大小对于甜桃的育种至关重要.
KLUH/CYP78A5基因调节植物中的种子和果实大小,但其机制尚未完全理解.

研究的目的:

调查PavKLUH在甜桃水果大小调节中的作用.
为了确定控制甜桃果实大小的分子机制.

主要方法:

在Arabidopsis.中PavKLUH的宫外过度表达.
病毒诱导的PavKLUH在甜桃中的基因沉默.
酵母单杂交 (Y1H) 试验,电泳性移动性转移试验和双化酶记者分析以研究PavRAV2和PavKLUH的相互作用.

主要成果:

过度表达PavKLUH会增加Arabidopsis silique和种子的大小.
在甜桃中抑制PavKLUH可以通过限制中骨细胞扩张来减少水果大小.
一个AP2/ERF转录因子PavRAV2直接结合并抑制PavKLUH促进体活性.
沉默PavRAV2导致了较大的甜桃果实,由于中骨细胞扩张的增加.

结论:

PavRAV2直接调节了PavKLUH的表达,影响了甜桃水果的大小.
这项研究阐明了一种涉及RAV转录因子和CYP78A家族成员在水果发育中的新信号通路.

关键词:

帕夫克卢赫 (PavKLUH) 是一个古老的城市.这就是PavRAV2的原因.果实大小果实大小果实大小基因沉默是对基因进行沉默的方法.活动主办人活动主办人甜蜜的桃甜蜜的桃

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