作为Clavibacter capsici的假定无形作用因子,ChpG作为植物中的过敏反应和病毒性 (Hrv) 蛋白
Eom-Ji Oh1, In Sun Hwang2, Choon-Tak Kwon3
1Plant Immunity Research Center, Seoul National University, Seoul 08826, Korea.
Molecular plant-microbe interactions : MPMI
|December 26, 2023
概括
克拉维巴克特菌是一种细菌.
科学领域:
- 植物病理学 植物病理学
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
背景情况:
- 克拉维细菌分泌的植物毒性和非宿主过敏反应 (HR) 的apoplastic作用因子.
- 克拉维巴克特 (Clavibacter capsici) 的 ChpG 蛋白质对于胡叶的死亡发生至关重要.
研究的目的:
- 为了研究CHpG和paralogs在尼古西亚烟草中HR诱导中的功能.
- 阐明特定氨基酸残留在ChpG的HR诱导和毒性活动中的作用.
主要方法:
- 生成与烟草PR1b信号序列融合的全长和信号删除 (ΔSP) 成熟蛋白质形式.
- 尼古提亚烟草中克拉维巴克特蛋白的表达和功能分析,用于HR诱导.
- 基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因
- 将氨酸替代的chpG基因转化为Clavibacter capsici,以评估病毒性恢复.
主要成果:
- 全长度和 ΔSP ChpG,以及 ΔSP ChpE 和 Pat-1,触发了尼古西亚烟草中的 HR.
- 在ChpG中的特定氨基酸替代物消除或降低了HR诱导活性.
- 氨酸替代的chpG变体未能恢复胡的死发育.
结论:
- 作为宿主植物的毒性因子和非宿主植物的HR诱导者,ChpG表现出双重功能.
- 催化三和二硫化物桥梁残留物对于ChpG的HR诱导活性至关重要,但对于胡中死的发展至关重要.
- 像ChpG这样的Clavibacter细胞增生因子被认为是过敏反应和毒性 (Hrv) 蛋白质,类似于阴性细菌中的Hrp蛋白质.
关键词:
克拉维巴克特 (Clavibacter) 是一个细菌.这就是为什么Hrv Hrv作为一个apoplastic效应器.细菌性癌症是一种细菌性癌症.过敏反应是一种过敏反应.血清蛋白质酶是一种血清蛋白质酶.更多相关视频
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