从临床样本中分离出的Candida haemulonii复合物的鉴定和抗真菌敏感性测试
Carlos Ruiz de Alegría-Puig1, Jesús Rodríguez-Lozano2, Jesús Agüero-Balbin2,3
1Microbiology Service, University Hospital Marqués de Valdecilla-IDIVAL-CIBERINFEC, Avda. Valdecilla s/n, CP 39008, Santander, Spain. carlosrdap@hotmail.com.
Folia microbiologica
|December 26, 2023
概括
在Candida haemulonii复合体显示显著的抗真菌耐药性. 与API方法不同,Vitek MS,Vitek MS PRIME和Vitek2系统可靠地识别这个复合体,有助于临床诊断.
科学领域:
- 医学真菌学 医学真菌学
- 临床微生物学 临床微生物学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 在Candida haemulonii复合体中,包括C. haemulonii,C. duobushaemulonii,以及C. haemulonii var. 由于其显著的抗真菌耐药性,越来越多地被认为是脆弱的.
- 尽管它在临床样本中并不普遍,但它的耐药性特征使得准确的识别对于有效的治疗策略至关重要.
研究的目的:
- 评估Candida haemulonii复合体的各种识别方法的性能.
- 评估商业和参考方法对这种物种的抗真菌敏感性测试 (AST) 的准确性.
主要方法:
- 通过生物化学和蛋白质组技术确定了10种Candida haemulonii复合物的分离物.
- 使用商业套件和参考方法进行了抗真菌敏感性测试.
- 使用矩阵辅助激光脱吸/电离飞行质谱 (MALDI-TOF MS) 系统 (Vitek MS和Vitek MS PRIME) 和Vitek2进行识别.
主要成果:
- MALDI-TOF MS (Vitek MS和Vitek MS PRIME) 和Vitek2正确识别了Candida haemulonii复合体,MALDI-TOF MS能够在亚种级别进行识别.
- 该API方法未能准确识别该综合体内的物种.
- 在抗真菌敏感性测试的商业和参考方法之间观察到强烈的相关性.
- 抗真菌敏感性测试证实了对安福特瑞辛B和醇的耐药性.
结论:
- 维特克MS,维特克MS PRIME和维特克2是识别Candida haemulonii复合物的可靠系统,而API32C方法不是.
- MALDI-TOF MS在复合体内提供了亚种级别识别的优势.
- 商业方法适用于对Candida haemulonii复合物的抗真菌敏感性测试,确认其耐药性模式.
相关概念视频
01:27Cryptococcal Meningitis
Cryptococcal meningitis is a life-threatening opportunistic infection predominantly associated with HIV/AIDS, accounting for over 100,000 deaths annually worldwide. However, it also affects individuals with other forms of immunosuppression, including those undergoing immunosuppressive therapy, organ transplant recipients, patients with innate immunodeficiencies, and individuals with hematological disorders. The infection is caused mainly by Cryptococcus neoformans and Cryptococcus gattii,...
01:20Candidiasis
Candidiasis is a fungal infection caused by opportunistic species of Candida. It can affect various anatomical sites, including the skin, oral cavity, nails, and genitourinary tract. Among its forms, vaginal candidiasis is the most common type of mucosal infection. It typically results from the overgrowth of Candida albicans in the vaginal mucosa. Under normal conditions, C. albicans exists as a commensal organism within the vaginal microbiota, regulated by the dominance of lactobacilli, which...
01:15Antifungal Agents
Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...