阿特拉提洛德斯 (Atractylodes) 巨头多糖的表面形态及其对PCV2复制的抑制作用
Changxi Qi1, Changyang Lv1, Menglin Jiang1
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
Journal of the science of food and agriculture
|December 27, 2023
概括
来自Atractylodes macrocephala Koidz. 的总多糖. (PAMK) 有效地抑制了猪肉循环病毒2型 (PCV2) 复制. 通过抑制Pink 1/Parkin通路,PAMK可以缓解PCV2诱导的线粒,从而提供潜在的抗病毒免疫疗法.
科学领域:
- 兽医免疫学 兽医免疫学
- 分子病毒学分子病毒学
- 自然产品化学 自然产品化学
背景情况:
- 猪病毒2型 (PCV2) 感染在猪业中造成重大经济损失,原因是免疫抑制和二次感染.
- 吸引类巨头科伊兹科伊兹. 聚糖化物 (PAMK) 在增强免疫力和抗病能力方面表现出潜力,这表明抗病毒应用.
研究的目的:
- 调查PAMK在缓解PCV2感染方面的疗效.
- 阐明PAMK对PCV2.2的抗病毒活性背后的分子机制.
主要方法:
- 描述PAMK的形态和结构.
- 评估PCV2诱导的自标志物 (LC3-II,p62) 和病毒载量的变化.
- 评估PAMK对线粒体途径 (PINK1,帕金,TOMM20),活性氧物种和线粒体功能的影响.
- 病毒Cap蛋白表达和病毒拷贝数量的量化.
主要成果:
- 帕姆克表现出一个紧的,多孔的和聚合的形态,没有三重螺旋形状.
- PCV2感染增加了自标志物,病毒Cap蛋白和病毒拷贝数.
- 帕姆克治疗抑制了PCV2复制,减轻了PCV2诱导的自,并减弱了PINK1上调和TOMM20下调.
- PAMK恢复了线粒体功能,减少了活性氧物种,并减少了病毒Cap蛋白的表达.
结论:
- 通过抑制Pink 1/Parkin通路,PAMK通过缓解PCV2诱导的线粒来抑制PCV2复制.
- 这些发现表明PAMK是预防和治疗PCV2的潜在治疗剂.
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