三维水凝与人类沃顿果衍生的介质细胞干细胞朝着核的核的利基
Isma Liza Mohd Isa1,2,3, Izzat Zulkiflee2, Raed H Ogaili1
1Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Frontiers in bioengineering and biotechnology
|December 27, 2023
概括
这项研究开发了一种氨酸/II型原蛋白水凝,用于再生椎间盘. 这种生物材料成功地支持了人类沃顿.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 基于细胞外矩阵 (ECM) 的生物材料和干细胞为脊椎间盘 (IVD) 再生提供了一个有前途的战略.
- 了解人类沃顿果衍生-介质干细胞 (hWJ-MSCs) 在3D微环境中分化成核 (NP) 样细胞至关重要.
- 目前在模仿本地NP微环境以有效修复内源性组织方面存在局限性.
研究的目的:
- 使用II型原蛋白 (COLII) 和氨酸 (HA) 开发3D生物工程水凝,以模仿原生NP微环境.
- 定制水凝系统以支持hWJ-MSCs向NP类细胞的分化.
- 评估HA/COLII水凝作为用于IVD再生的细胞输送系统的适用性.
主要方法:
- 制造3D水凝使用COLII丰富HA,不同的生物大分子度.
- 描述水凝的特性,包括降解,稳定性和胀.
- 在水凝中封装hWJ-MSC,以评估细胞活力,形态和分布.
主要成果:
- 成功制造了COLII/HA水凝,保持了3D结构,并证明了可调节的膨胀能力,含有更高的HA含量.
- 水凝在一个月内表现出超过60%的水解稳定性,在第14天降解程度增加.
- 封装的hWJ-MSC显示圆形形态,均分布和增强细胞生长直到14天.
结论:
- HA/COLII水凝提供最佳的膨胀,稳定性和可降解性,模仿NP微环境.
- 水凝有效地引导hWJ-MSCs向NP表型.
- 这种HA/COLII水凝系统显示出作为脊椎间盘再生的先进细胞输送载体的潜力.
相关概念视频
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