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提供微RNA-200c的纳米颗粒抑制口腔状细胞癌

Q J Ding¹, M T Remy¹, C Upara¹

¹Iowa Institute for Oral Health Research, College of Dentistry, University of Iowa, Iowa City, IA, USA.

Journal of dental research

|December 27, 2023

概括

这项研究开发了一种使用碳酸纳米颗粒的新型基因疗法,用于输送用于治疗口腔癌的微RNA-200c (miR-200c). 在临床前模型中,该疗法有效抑制了口腔状细胞癌 (OSCC) 的生长和迁移.

科学领域:

生物医学工程生物医学工程
在瘤学瘤学.
基因治疗基因治疗

背景情况:

口腔状细胞癌 (OSCC) 是一种普遍存在的头癌,复发率,转移率和死亡率很高.
目前对OSCC的治疗结果仍然不理想,需要开发新的治疗策略.
微RNA (miR) -200c已经证明了瘤抑制性质,但其通过基因疗法在OSCC中的治疗应用尚未先前报告.

研究的目的:

研究使用碳酸 (CaCO3) 基纳米颗粒用于非病毒传递编码miR-200c的等离子体DNA (pDNA) 的可行性,以抑制OSCC生长.
评估基于CaCO3的纳米颗粒在传染OSCC细胞中具有编码miR-200c的pDNA的效率.
评估miR-200c过度表达对OSCC细胞增殖,迁移,瘤基因表达和体内瘤生长的影响.

主要方法:

以CaCO3为基础的纳米颗粒配制了不同比例的CaCO3和胺硫酸盐 (PS),以优化OSCC细胞中的pDNAmiR-200c转染效率.
在体外测量了miR-200c过度表达对OSCC细胞增殖和迁移的影响.
在实体中,使用细胞系衍生异种移植 (CDX) 来评估瘤生长,在局部给予CaCO3 / pDNA miR-200c纳米复合体后,在小鼠中进行了评估.

主要成果:

基于CaCO3的纳米颗粒证明了对pDNAmiR-200c的转染效率的提高,CaCO3与PS的比率显著影响了这一过程.
过度表达miR-200c显著抑制OSCC细胞的增殖,迁移和瘤基因表达.
在小鼠CDX模型中,局部给药CaCO3/pDNAmiR-200c纳米复合体有效抑制了瘤生长.

结论:

这种CaCO3/pDNAmiR-200c纳米复合体显示出作为一种抑制OSCC进展的非病毒基因传递系统的显著潜力.
这种新的方法可能为减少口腔癌复发和改善临床结果提供了一个有希望的策略.
需要进一步进行全面的临床前研究,以证实这种CaCO3/pDNA miR-200c系统在各种模型中的安全性和有效性.

关键词:

基于CaCO3的纳米粒子这是OSCCCC的OSCC.在 miR-200c 中使用.转换效率效率是指转换的效率.瘤生长,异种移植

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