ангиотензин-(1-7) 在盐度应激下调节Trachemys scripta elegans的精子生成中起着重要的作用
Na Li1, Qiongyu Zhang1, Shiyu Dai1
1Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China.
The Journal of experimental biology
|December 27, 2023
概括
高度的水对乌的丸产生负面影响,导致炎症和生殖细胞丧失. 然而,血管素-1-7可以防止这些影响,为盐度引起的生殖问题提供潜在的治疗标.
科学领域:
- 生殖生物学 生殖生物学
- 环境毒理学环境毒理学
- 内分泌学 在内分泌学.
背景情况:
- 水的盐度升高对淡水水生动物的生殖健康构成威胁.
- 氨酸- ангиотензин系统 (RAS) 在调节精子生成中起着至关重要的作用,使其成为理解生殖功能障碍的关键研究领域.
- 之前的研究表明,红耳滑鼠 (Trachemys scripta elegans) 在盐水环境中的弹性.
研究的目的:
- 研究度暴露对雄性红耳滑鼠丸精子生成的慢性影响.
- 阐明潜在的机制,特别是胺-血管素系统 (RAS) 途径的参与,度诱导的丸变化.
- 评估 ангиотензин-(1-7) 在减轻度诱导的丸损伤中的潜在保护作用.
主要方法:
- 雄性红耳滑鱼 (Trachemys scripta elegans) 被暴露在淡水 (对照) 中,5 PSU 和10 PSU 盐水中一年.
- 精子生成的评估,包括生殖细胞的数量和炎症和亡基因的表达 (TNF-α,IL-12A,IL-6,TGF-β1,Bcl-6,Bax,caspase-3).
- 评估氨酸-血管氨系统 (RAS) 途径,特别是测量血管氨-1-7水平及其抑制剂 (A-779) 的作用.
主要成果:
- 暴露于10 PSU盐度显著减少丸生殖细胞数量和诱导炎症,由高的促炎基因表达体现.
- 在暴露于10 PSU的海中, ангиотензин-(1-7) 水平显著增加,这表明其具有补偿性抗炎和抗亡作用.
- 抑制 ангиотензин-(1-7) 加剧了盐度诱导的炎症和亡,同时阻断了保护基因 (TGF-β1,Bcl-6) 的上调.
结论:
- 长期暴露于盐度会通过炎症和亡对红耳滑动动物的丸精子生成产生负面影响.
- 胺血管素-(1-7) 通过调节炎症和亡途径,在减弱度诱导的丸功能障碍方面发挥着显著的保护作用.
- 向血管素-1-7通路为预防或治疗水生动物盐度诱导的生殖功能障碍提供了一种新的治疗策略.
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