聚二甲基西洛与基于Diazirine的链接剂的功能化,用于共价蛋白固定
Jie Li1, Liting Bi2, Stefania F Musolino2
1School of Biomedical Engineering, McMaster University, Hamilton, Ontario L8S 4L2, Canada.
ACS applied materials & interfaces
|December 27, 2023
概括
新合成的迪亚齐林分子能够在聚甲基素 (PDMS) 表面上增强生物分子固定. 这种新的,非破坏性的表面修饰技术改善了生物医学应用中的蛋白质附着和稳定性.
科学领域:
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
- 表面化学 表面化学
背景情况:
- 生物分子附着在固体支上对于生物传感器和植入物等生物医学设备至关重要.
- 聚甲基 (PDMS) 是由于其有利的特性而在这些应用中广泛使用的材料.
- 在PDMS上增强生物分子固定是提高设备性能至关重要的.
研究的目的:
- 开发一种新的,非破坏性的方法来修改PDMS表面,以改善生物分子固定.
- 为PDMS表面功能化合成和利用新的迪亚齐林分子.
- 评估蛋白质附着在修改后的PDMS表面的效率和稳定性.
主要方法:
- 使用通过C-H插入新合成的迪亚齐林分子对PDMS的表面修饰.
- 热或紫外线激活用于迪亚齐林-PDMS反应.
- 表面表征技术以确认PDMS修改.
- 使用-125放射标记对固定蛋白质 (牛血清白蛋白和免疫球蛋白G) 的量化.
- 同焦显微镜可视化蛋白质分布和链接器透.
主要成果:
- 通过使用迪亚齐林分子实现了PDMS表面的成功和快速功能化.
- 观察到显著改善的稳定性和固定蛋白质 (BSA和IgG) 的强固附着.
- 多个西林分子和激活方法证明了有效性.
- 同焦点显微镜提供了关于固定IgG和链接器透的分布的见解.
结论:
- 这项研究提出了一种有前途的新方法,用于功能化PDMS表面,以增强生物分子固定.
- 基于迪亚齐林的表面修饰技术可以改善蛋白质的附着性和稳定性.
- 这种方法在各种诊断和医疗设备中具有潜在的应用,包括微流体设备和免疫测试.
相关概念视频
01:01Phosphodiester Linkages
Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
01:33Carboxylic Acids to Methylesters: Alkylation using Diazomethane
Carboxylic acids react with diazomethane in an ether solvent via alkylation at the carboxylate oxygen atom to give methyl esters of the corresponding acid with excellent yields.
01:16Structure of Conjugated Dienes
Introduction
Conjugated dienes are compounds characterized by the presence of alternating double and single bonds. In a conjugated system like 1,3-butadiene, the unhybridized 2p orbital on each carbon overlaps continuously, allowing the π electrons to be delocalized across the entire molecule. In contrast, this type of overlap does not occur in cumulated and isolated dienes, such as 2,3-pentadiene and 1,4-pentadiene, respectively. Instead, the π electrons remain localized between the double...
Conjugated dienes are compounds characterized by the presence of alternating double and single bonds. In a conjugated system like 1,3-butadiene, the unhybridized 2p orbital on each carbon overlaps continuously, allowing the π electrons to be delocalized across the entire molecule. In contrast, this type of overlap does not occur in cumulated and isolated dienes, such as 2,3-pentadiene and 1,4-pentadiene, respectively. Instead, the π electrons remain localized between the double...
01:19Diazonium Group Substitution: –OH and –H
Nitrous acid, a weak acid, is prepared in situ via the reaction of sodium nitrite with a strong acid under cold conditions. This nitrous acid prepared in situ reacts with primary arylamines to form arenediazonium salts. Such reactions are known as diazotization reactions. As shown in Figure 1, the formation of arenediazonium salts begins with the decomposition of nitrous acid in an acidic solution to give nitrosonium ions.
01:11Aryldiazonium Salts to Azo Dyes: Diazo Coupling
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para position.
01:371° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism
Nitrous acid is a relatively weak and unstable acid prepared in situ by the reaction of sodium nitrite and cold, dilute hydrochloric acid. In an acidic solution, the nitrous acid undergoes protonation when it loses water to form a nitrosonium ion—an electrophile. Nitrous acid reacts with primary amines to give diazonium salts. The reaction is called diazotization of primary amines.