转录因子VviWOX13C通过直接激活葡萄 (Vitis vinifera L) 中的VviEXPA37/38/39来调节果
Yadan Sun1, Yihan Yue1, Xufei Li1
1College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.
葡萄扩展基因,包括VviEXPA37,VviEXPA38和VviEXPA39,对于果至关重要. VviWOX13C直接激活这些扩展基因,突出显示它们在葡萄发育中的调节作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 植物生理学 植物生理学
背景情况:
- 扩素是一种非酶的细胞壁蛋白,对细胞扩大至关重要.
- 了解水果组中的扩散基因功能是有限的.
- 葡萄藤 (Vitis vinifera L.) 的果是一个关键的发育过程.
研究的目的:
- 为了识别和表征葡萄中的扩散基因家族.
- 为了研究水果组中的特定扩散基因的作用.
- 阐明VviWOX13C对扩大基因表达的调节机制.
主要方法:
- 在Vitis vinifera中扩展基因的全基因组识别.
- 遗传学分析和扩散的域识别.
- 转录组测序用于分析在CPPU治疗下基因表达.
- 电泳运动移位试验 (EMSA) 和双化酶试验以确认转录调节.
主要成果:
- 在葡萄基因组中确定了29个扩展基因,分为四个子家族.
- VviEXPA37,VviEXPA38和VviEXPA39的表达被CPPU上调.
- VviWOX13C被确定为VviEXPA37,VviEXPA38和VviEXPA39的直接转录激活剂.
- VviWOX13C直接与这些扩展基因的促进体结合.
结论:
- VviWOX13C在葡萄中扩张介导的果实中起着关键的调节作用.
- 这项研究揭示了一种涉及VviWOX13C和特定扩展基因的新型调节途径.
- 为进一步研究果实发育中的扩大基因功能和调节提供了基础.
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