在全球废水中Escherichia coli隔离物中具有抗性多样性
Pavithra Anantharaman Sudhakari1, Bhaskar Chandra Mohan Ramisetty1
1Laboratory of Molecular Biology and Evolution, 312@ASK1, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, India.
Microbial drug resistance (Larchmont, N.Y.)
|December 27, 2023
概括
抗菌素耐药性 (AMR) 基因存在于健康的青年废水中. 这表明抗生素耐药性基因 (ARG) "入侵"了人类微生物群,突出了关键的公共卫生问题.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生 公共卫生
- 基因组学就是基因组学.
背景情况:
- 抗菌素耐药性 (AMR) 是一个重大的全球健康威胁.
- 污水管理不善和全球旅行加快了多药耐药 (MDR) 菌株的传播.
- 对于社区一级的AMR流行情况的了解有限,特别是在健康人群中.
研究的目的:
- 来自健康的年轻人群体的废水中调查AMR大肠杆菌的存在和特征.
- 分析AMRE.E.的基因组和抗体. 它将大肠杆菌分离并与全球数据进行比较.
- 了解抗生素耐药性基因 (ARG) 在人类微生物组中的传播.
主要方法:
- 从当地废水中分离出的大肠杆菌AMR的表征.
- 抗性和含有等离子体的E.E.的量化 大肠杆菌.
- 选择的MDR和敏感菌株的全基因组测序.
- 与E.进行比较分析. 来自PATRIC数据库中的大肠杆菌基因组.
主要成果:
- 26%的E.I.的使用量. 当地废水中的大肠杆菌分离物具有耐药性,其中59%含有塑体.
- 环素,卡纳米辛和氨基醇是常见的耐药性目标;五个分离物是MDR.
- 整个基因组分析显示,全球E.E.之间的广泛相似性. 大肠杆菌分离物,表明广泛传播.
- 全球废水电阻组由ARG主导,对抗氨基糖化物,β-乳酸盐,硫胺胺和三甲.
- 在来自欧洲和南亚地区的MDR分离物中观察到胆固醇耐药性.
结论:
- 健康的年轻人群为废水提供ARG,这表明了废水的价值.
- 这是入侵的入侵.
- 的ARG进入人类微生物组.
- 全球传播和E.E.的殖民化. 大肠杆菌通过基因组相似性显而易见.
- 一种包括减少抗生素使用和改进废水管理在内的综合性方法对于打击AMR至关重要.
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