在Caenorhabditis elegans中的多能原体中,PBRM-1/PBAF受调节的基因在Caenorhabditis elegans中的多能原体中
Laura D Mathies1, Andrew C Kim1, Evan M Soukup1
1Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA.
G3 (Bethesda, Md.)
|December 27, 2023
概括
聚菌相关的BAF (PBAF) 染色体重塑剂促进Caenorhabditis elegans体性淋巴前体 (SGP) 细胞命运. 失去PBAF会影响肌肉基因表达,并确定SGP/hmc细胞命运决定的候选调节者.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 阴性淋巴前体 (SGP) 是多强的原始体,在C. elegans中产生成人生殖系统.
- 聚烯相关的BAF (PBAF) 染色体重塑复合物对于维持SGP多能性至关重要.
- 完全失去PBAF是致命的,需要有条件的基因删除策略.
研究的目的:
- 调查PBAF在中皮细胞的下游标,包括SGP.
- 确定由PBAF调节的基因,这些基因控制SGP和头部中皮细胞 (hmc) 的命运.
- 了解PBAF在肌肉发育和SGP分化中的作用.
主要方法:
- 利用Cre/lox重组和GFP纳米体导向蛋白质降解条件消除PBRM-1,PBAF子单元.
- 在缺乏PBRM-1的L1幼虫中皮上进行RNA测序.
- 使用光报告器验证了候选基因调节.
主要成果:
- 在PBRM-1删除后,在中皮体中鉴定了1,955个差异表达的转录.
- 发现肌肉细胞功能基因的丰富,在pbrm-1突变体中表达较低,表明PBAF促进肌肉分化.
- 发现了125个富含SGP和53个富含HMC的候选基因,参与SGP/HMC命运决定.
结论:
- PBAF在调节中皮层基因表达,包括肌肉发育方面发挥着重要作用.
- 通过差异基因调节,PBAF对于控制SGP/hmc细胞命运决定至关重要.
- 由PBAF调节的特定基因,如hsp-12.3,是理解SGP发育和分化的关键.
关键词:
这里是C. elegans.在pbrm-1中使用.在PBAF中,PBAF是PBAF.这是SGP的SGP.在SWI/SNF染色体重塑过程中.不同的基因表达.在此之前,HMC是HMC.多强的原始基因.肌肉 肌肉 肌肉 肌肉 肌肉更多相关视频
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