circMORC3编码的新型蛋白质通过与宿主基因MORC3的协同作用来负面调节抗病毒免疫力
Linchao Wang1, Weiwei Zheng1, Xing Lv1
1Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
PLoS pathogens
|December 27, 2023
概括
发现一种新型的循环RNA (circRNA),circMORC3及其编码 (MORC3-84aa) 抑制了鱼类的抗病毒免疫力. 它们与宿主基因MORC3协同工作,帮助病毒逃脱免疫检测.
科学领域:
- * 分子生物学 * 分子生物学
- * 免疫学 免疫学
- * 水生动物健康
背景情况:
- * 循环RNAs (circRNAs) 的蛋白质编码潜力是一个新兴的研究领域.
- * 在远鱼抗病毒天生的免疫力中蛋白质编码circRNAs的功能仍然在很大程度上未被探索.
- *了解这些机制对于制定对鱼类病毒感染的策略至关重要.
研究的目的:
- * 识别和描述涉及远端抗病毒免疫的新型蛋白质编码circRNAs.
- *阐明circMORC3及其编码的调节抗病毒反应的机制.
- * 调查circMORC3及其宿主基因MORC3在病毒免疫逃逸中的协同作用.
主要方法:
- * 来自Miichthys miiuy. 的circMORC3的鉴定和特征.
- *分析circMORC3和MORC3-84aa对抗病毒细胞因子表达和信号通路 (TRIF,IRF3,NF-κB) 的影响.
- *研究MORC3-84aa和TRIF之间的相互作用,包括无处化和自降解试验.
主要成果:
- *发现了一种新型的circRNA,circMORC3,它编码了一个84氨基酸,MORC3-84aa.
- *MORC3-84aa显著抑制了TRIF介导的IRF3和NF-κB通路的激活,抑制了由Siniperca chuatsi rhabdovirus (SCRV) 诱导的抗病毒细胞因子表达.
- *MORC3-84aa与TRIF直接相互作用,通过K6结合的无化促进其自降解,从而抑制I型干扰素信号传递. 主体基因MORC3也抑制了IRF3的表达和酸化.
结论:
- *circMORC3及其编码的MORC3-84aa作为远鱼的抗病毒先天免疫力的负调节剂.
- *MORC3-84aa通过增强的泛化和自降解来向TRIF介导的信号通路来抑制病毒复制.
- *circMORC3和宿主基因MORC3在促进病毒免疫逃逸方面表现出协同作用.
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