由线粒体ROS触发的氨基基基分子释放的甲
Jing Liu1,2, Pu Yan1,2, Xiangjun Liu1,2
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
ACS applied bio materials
|December 27, 2023
概括
研究人员开发了一种用于线粒体的新ROS触发分子释放系统. 该系统使用双向分子释放核染料以应对活性氧物种 (ROS),从而实现细胞监测.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 分子成像学分子成像学
背景情况:
- 线粒体是活性氧物种 (ROS) 生产的关键地点.
- 细胞中受控的分子释放对于生物研究和治疗干预至关重要.
- 目前用于活细胞中分子过程的时空控制的方法是有限的.
研究的目的:
- 开发一种用于ROS触发分子释放的新策略,特别是在线粒体内.
- 创建一个光探测器,用于监测活细胞中的线粒体ROS水平.
- 调查IR-780作为内源ROS响应分子释放平台的潜力.
主要方法:
- 通过连接线粒体染料 (IR-780) 和核染料 (NH2-TO) 来设计和合成双向分子 (IRTO).
- 使用光成像的IRTO的细胞吸收和局部化研究.
- 研究IRTO对不同水平的ROS (由H2O2,LPS引起) 和ROS抑制剂 (NAC,MitoQ) 的反应.
- 监测NH2-TO从线粒体到核的时空释放.
主要成果:
- IRTO成功地积聚在活细胞的线粒体中,呈现出红色光.
- 在与线粒体ROS反应后,NH2-TO从线粒体释放出来,并定位在核中,显示绿色光.
- NH2-TO的释放被ROS诱导剂 (H2O2,LPS) 加速,并被ROS清理剂 (NAC,MitoQ) 抑制.
- 光成像允许通过连续的红色到绿色光转移来可视化线粒体ROS水平.
结论:
- IRTO作为一种有效的光探测器来监测活细胞中的线粒体ROS.
- 开发的策略证明了从线粒体释放内源性ROS触发分子的可行方法.
- IR-780显示出作为开发用于生物应用的ROS响应分子平台的核心组件的前景.
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