来自Acinetobacter baumannii LUH5551的多糖体的修订结构被赋予K63型囊多糖体
Nikolay P Arbatsky1, Anastasiya A Kasimova1, Alexander S Shashkov1
1N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Carbohydrate research
|December 27, 2023
概括
来自Acinetobacter baumannii LUH5551的K63囊多糖含有一种新的军团胺酸衍生物,Leg5Ac7R. 这种结构,通过先进的光谱学证实,不同于以前的O24表征.
科学领域:
- 微生物学 微生物学
- 碳水化合物化学 碳水化合物化学
- 基因组学就是基因组学.
背景情况:
- 宝曼尼菌 (Acinetobacter baumannii) 产生囊多糖 (CPS),这对其毒性和免疫逃逸至关重要.
- 之前从O24分离物中对K63囊的结构分析报告了一种不同的军团氨基酸衍生物.
研究的目的:
- 为了重新阐明来自Acinetobacter baumannii的K63囊多糖的精确结构,我们分离了LUH5551.1.
- 为了将阐明的CPS结构与其生物合成的潜在遗传决定因素相关联.
主要方法:
- 糖分分析 糖分分析
- 史密斯降解的降解方式
- 1D和2D1H和13C的NMR光谱学
- 对KL63基因集群的生物信息分析.
主要成果:
- 该K63囊包括线性四糖体单元,其中含有5,7-米诺-3,5,7,9-四度氧-d-糖-d-银酸-非-2-氨酸 (莱吉亚胺酸) 的衍生物,被指定为Leg5Ac7R.
- Leg5Ac7R与 (S) - 3 - 基布他或乙基以大约1:1的比例被乙基化.
- 在KL63位点内的lgaABCHIFG基因模块负责Leg5Ac7R生物合成,而KL63编码的葡萄糖转移酶和Wzy聚合酶调解了特定的链接.
结论:
- 来自Acinetobacter baumannii LUH5551的K63囊多糖的结构已经得到了明确的阐明,揭示了一种新的军团氨基酸衍生物.
- 这种特定的囊型多糖体结构生物合成的遗传基础是由KL63基因集群的存在和功能证实的.
关键词:
这种细菌是Acinetobacter baumannii.囊中的多糖化物.这里是K locus.K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63 K63LUH5551 一个小说更多相关视频
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