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百合花的功能分析短植物阶段在开花过渡期和花发育中的正向体

Xiaomei Wu¹, Wu Ling², Yusha Pan¹

¹Hunan Mid-subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center, College of Horticulture, Hunan Agriculture University, Changsha, 410128, China.

Plant physiology and biochemistry : PPB

|December 27, 2023

概括

的SVP基因 (LfSVP) 作为一个开花抑制剂. 沉默LfSVP加速开花并改变百合的花朵发育,有助于分子繁殖.

科学领域:

植物分子生物学植物分子生物学
关于开花的规定开花规则
装饰园艺装饰园艺

背景情况:

百合 (百合) 是一个具有商业意义的球形花科.
了解开花机制对于调节百合花的开花至关重要.
MADS-box SHORT VEGETATIVE PHASE (SVP) 基因影响植物的开花过渡和花器官发育.

研究的目的:

在Lilium × formolongi (LfSVP) 中识别和描述SVP正义词.
研究LfSVP在调节花的开花时间和花器官分化中的作用.
探索LfSVP与其他花调节者的潜在相互作用.

主要方法:

遗传学分析以确定LfSVP与Arabidopsis SVP的关系.
对LfSVP的组织特异和阶段依赖的表达模式的分析.
通过阿拉比多普西斯的过度表达和L. × formolongi.中的基因沉默 (烟草虫病毒) 的功能分析.
酵母和烟草细胞测定用于研究LfSVP和APETALA1 (AP1) 之间的蛋白质蛋白相互作用.

主要成果:

LfSVP表达在叶子中达到峰值,在叶片中低,在花诱导和花芽阶段的转录高.
在阿拉比多普西斯中LfSVP的过度表达导致花期延迟和花器官缺陷.
在L. × formolongi中,对LfSVP的基因沉默导致更早的开花和花器官缺陷.
发现LfSVP与L. ×formolongi AP1 (LfAP1) 相互作用.

结论:

LfSVP 作为百合花的开花抑制剂.
LfSVP参与调节花器官分化,可能通过与LfAP1.1的相互作用.
结果为花品种的分子育种提供了洞察力,这些品种具有受控的开花周期和多样化的花型.

关键词:

花的发展花的发展.花的诱导花的诱导百合花 × 形式隆基短暂的植物生长阶段

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