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基于纳米孔的元基因组学揭示了欧洲的新瑞克特菌

Suppaluck Polsomboon Nelson¹, Koray Ergunay², Brian P Bourke¹

¹Walter Reed Biosystematics Unit (WRBU), Smithsonian Institution, Museum Support Center, Suitland, MD, USA; Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, USA; Smithsonian Institution, Department of Entomology, National Museum of Natural History (NMNH), Washington, DC, USA.

Ticks and tick-borne diseases

|December 27, 2023

概括

非向的元基因组测序提供了一种全面的方法来识别传播细菌,包括Rickettsia物种,揭示了比传统PCR方法更广泛的病原体谱. 这种先进的技术增强了对的各种细菌感染的检测.

科学领域:

兽医微生物学兽医微生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

传播的细菌感染,特别是病,由于复杂的微生物和载体相互作用,存在诊断挑战.
准确识别这些病原体对于了解疾病传播和公共卫生影响至关重要.

研究的目的:

用纳米孔技术对比非向的元基因组测序 (NS) 与用于在中检测瑞克菌的常规PCR试验进行比较评估.
评估来自波兰和保加利亚的现场采集的虫中虫传播细菌的频谱,包括Rickettsia物种.

主要方法:

对310只子 (Ixodes ricinus,Dermacentor reticulatus等) 的分析来自波兰和保加利亚,在55个池中加工.
总核酸提取,然后进行纳米孔测序 (NS) 和PCR分析,以Rickettsia ompA/gltA基因为目标.
用 mutS, uvrD, argS 和 virB4 序列进行植物遗传学分析,以确认瑞克特西亚物种.

主要成果:

在47.3%的池中,NS发现了Rickettsia,而PCR在54.5%的池中检测到它们.
最常见的物种是Rickettsia asiatica (27.2%) 和Rickettsia raoultii (21.8%);R. asiatica最近在欧洲被记录.
此外,NS还检测到Anaplasma phagocytophilum,Coxiella burnetii,Neoehrlichia mikurensis和各种内生生物,在16.4%的池中观察到共感染.

结论:

与向PCR相比,非向的元基因组测序为识别传播的细菌病原体提供了强大的和更广泛的诊断能力.
这种方法有助于发现新型病原体,并全面了解的微生物组合.
这项研究强调了NS对传播细菌病的监测和准确诊断的实用性.

关键词:

保加利亚保加利亚保加利亚保加利亚纳米孔测序的测序波兰波兰波兰波兰里克特西亚 (Rickettsia) 是一个古老的植物.子子子子

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