脊椎网状细胞中的动态引力:2D培养模型用于正常和眼状态
Alireza Karimi1, Mini Aga2, Taaha Khan2
1Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, OR, USA; Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR, USA.
Acta biomaterialia
|December 27, 2023
概括
与健康个体相比,青光眼患者在带状网状细胞中表现出更大的引力. 这项研究开发了一种新的2D模型来测量这些3D细胞力量,有助于眼研究.
科学领域:
- 生物医学工程 生物医学工程
- 细胞机械生物学 细胞机械生物学
- 眼科医生 眼科 眼科
背景情况:
- 青光眼与眼内压力升高 (IOP) 有关,导致状眼网 (TM) 细胞功能障碍和细胞外矩阵 (ECM) 刚性增加.
- TM细胞与ECM有动态相互作用,感知并运用影响水性幽默外流阻力的力.
研究的目的:
- 开发和验证一个2D体外细胞培养模型,用于量化人类TM细胞的3D,深度依赖的引力.
- 为了比较正常和眼的人类TM细胞在可变形基质上所施加的生物力.
主要方法:
- 人类TM细胞 (正常和眼) 在聚烯胺 (PAM) 凝基板上培养,并嵌入了FluoSpheres.
- 现场共聚焦显微镜捕获了PAM凝内的FluoSpheres的3D位移.
- 根据FluoSphere位移和已知的PAM凝刚度计算了引力和细胞应变.
主要成果:
- 玻璃眼TM细胞产生了比正常TM细胞大得多的3D引力,深入基质超过10μm.
- 在TM细胞中压力纤维的形成随着基质刚度的增加而增加,但在更高的刚度水平 (20-80 kPa) 降低.
结论:
- 开发的2D模型有效量化了TM细胞中的3D细胞力量,提供了对青光眼机械生物学的见解.
- 改变TM细胞中的细胞力量和生物力学特性是格劳科马病变的关键因素和水性幽默流出阻力.
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