阿贝尔莫斯克斯 (Abelmoschus) 马尼霍特 (L.) 医学.医药. 种子通过调节JAK2/STAT3信号通路来缓解类风湿性关节炎
Yiwen Tao1, Jia Liu1, Mengjia Li1
1School of Ethnic Medicine and Meishan Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
Journal of ethnopharmacology
|December 27, 2023
概括
阿贝尔莫斯库斯 (Abelmoschus) 麻豆种子 (AMS) 通过调节JAK2/STAT3通路,有效地降低了大鼠的类风湿性关节炎 (RA) 炎症. 这项研究确定了关键活性化合物及其机制,建议AMS作为RA的潜在治疗方法.
科学领域:
- 药理学和传统医学的医学.
- 免疫学和炎症研究研究
- 自然产品化学 自然产品化学
背景情况:
- 阿贝尔莫斯克斯 (Abelmoschus) 马尼霍特 (L.) 医学医学 种子 (AMS) 是西藏的一种草药,含有强效的黄类和类糖化物,如奎尔丁.
- 虽然AMS表现出抗风湿性关节炎 (RA) 的特性,但其潜在的药理机制在很大程度上仍未被阐明.
- 本研究研究了AMS在RA背景下的活性成分和治疗机制.
研究的目的:
- 为了确定AMS中关键的活性化学成分,负责其抗RA作用.
- 阐明AMS在RA上发挥治疗作用的药理机制.
- 为了验证JAK2/STAT3信号通路在AMS介导的RA治疗中的作用.
主要方法:
- 超高性能液体染色学-电喷电离-并联多阶段质谱学 (UPLC-ESI-IT-MSn) 用于分析AMS的化学成分.
- 用一种原诱导性关节炎 (CIA) 鼠标模型来评估AMS提取物的体内疗效.
- 分析了血清细胞因子水平 (IL-10,TNF-α,IL-1β,INF-γ),突组织蛋白质表达 (JAK2/STAT3通路),组织病理学和分子对接.
主要成果:
- 在AMS中发现了12种化合物,包括氨酸衍生物,可能调解其抗RA作用.
- 在CIA大鼠中,AMS治疗显著降低了关节胀,关节炎指数,并改善了体重.
- AMS调节了炎症性细胞因子配置,抑制了JAK2/STAT3通路蛋白 (JAK2,STAT3),并影响了与亡相关的蛋白 (Bcl-2/Bax,Caspase3).
结论:
- 阿贝尔莫斯克 (Abelmoschus manihot) 种子 (AMS) 在原诱导性关节炎的老鼠模型中显示出显著的疗效,可以改善关节炎症.
- 在RA中AMS的治疗效果与JAK2/STAT3信号通路的调制密切相关.
- 作为一种潜在的治疗药物,AMS有望成为管理与类风湿性关节炎相关的炎症的治疗药物.
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