Jove
Visualize
联系我们

相关概念视频

Proofreading01:31

Proofreading

6.3K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
6.3K
Mismatch Repair01:20

Mismatch Repair

4.9K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.9K
Overview of DNA Repair02:25

Overview of DNA Repair

31.0K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
31.0K
Base Excision Repair01:54

Base Excision Repair

22.4K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.4K
Genome Copying Errors02:46

Genome Copying Errors

4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

64.8K
64.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

New Analogs of the Compstatin Family of Clinical Complement Inhibitors with Low Picomolar Target Affinity.

Journal of medicinal chemistry·2026
Same author

Mitochondrial DNA replication is regulated by endoplasmic reticulum-mitochondrial contact sites, the mitochondrial calcium uniporter, and manganese.

Nucleic acids research·2026
Same author

Insights into the translational activation mechanisms of the COX1 mRNA in yeast mitochondria.

Journal of cell science·2025
Same author

Structural basis for promoter recognition and transcription factor binding and release in human mitochondria.

Molecular cell·2025
Same author

Structural Basis for Promoter Recognition and Transcription Factor Binding and Release in Human Mitochondria.

bioRxiv : the preprint server for biology·2025
Same author

Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins.

The Journal of biological chemistry·2025
Same journal

Large-scale discovery and annotation of substructure patterns in mass spectrometry profiles.

Nature communications·2026
Same journal

Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression.

Nature communications·2026
Same journal

A human-specific microRNA controls the timing of excitatory synaptogenesis.

Nature communications·2026
Same journal

An HMA-like integrated domain in the wheat tandem kinase WTK4 recognises an RNase-like pathogen effector.

Nature communications·2026
Same journal

Learning regularities in noise engages both neural predictive activity and representational changes.

Nature communications·2026
Same journal

The H3K4 methyltransferase KMT2D is an essential cofactor for GATA1 at erythroid gene enhancers.

Nature communications·2026
查看所有相关文章
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 7, 2025

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

9.7K

对于DNA校对的结构基础

Gina Buchel1, Ashok R Nayak1, Karl Herbine1

  • 1Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust St, Philadelphia, PA, 19107, USA.

Nature communications
|December 27, 2023
PubMed
概括
此摘要是机器生成的。

人类线粒体DNA聚合酶 (Polγ) 的校对涉及一个保存的"螺栓作用"机制. 这个过程反复地将聚合酶沿着DNA转移,而不会发生解离,从而使错误纠正对细胞活力至关重要.

更多相关视频

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

9.5K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

364

相关实验视频

Last Updated: Jul 7, 2025

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

9.7K
Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

9.5K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

364

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 遗传学 是一个遗传学.

背景情况:

  • DNA聚合酶 (DNAP) 校对对于保持基因组完整性和细胞活力至关重要.
  • 在DNAP校对过程中错误转移和DNA重新定位的精确机制仍然不完全理解.

研究的目的:

  • 阐明由人类线粒体DNA聚合酶 (Polγ) 进行DNA校对的逐步机制.
  • 揭示涉及从聚合酶到外核酶部位转移不匹配的结构动态.

主要方法:

  • 在校对过程中,高分辨率结构分析了人类Polg的九个不同的状态.
  • 功能测定和突变发生研究,以验证拟议的机制.

主要成果:

  • 详细的结构捕捉了关键事件:不匹配的基因识别,与聚合酶部位的解离,DNAP转位,DNA轨迹的变化和原料重新定位.
  • 有证据表明"螺栓作用"机制涉及代DNAP转位而无解离,从而促进了原料转移.
  • 病原性突变与涉及校对步骤的关键结构元素有关.

结论:

  • 一个保存的"螺栓作用"机制控制了人类Polγ的DNA校对,涉及连续的聚合酶转位.
  • 这种机制确保了有效的错误纠正,并突出了特定结构元素的功能重要性.
  • 了解这一过程,可以了解DNA复制的真实性和相关的遗传疾病.