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在反复流产中,MiR-3074-5p通过EIF2S1/GDF15通道调节热囊细胞功能

Jia-Xin Shi¹, Long Yang¹, Jie Gan¹

¹Shanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies, Medical School, Fudan University, Shanghai, 200237, China.

Reproductive sciences (Thousand Oaks, Calif.)

|December 27, 2023

概括

微RNA-3074-5p通过降低GDF15来调节异常性热囊细胞功能,可能导致重复性流产. 这一途径涉及针对EIF2S1,影响热囊细胞入侵和植入.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
遗传学遗传学是一个

背景情况:

外热囊细胞 (EVT) 功能障碍与早期怀孕失败有关.
经常性流产 (RM) 与改变的微RNA和生长分化因子15 (GDF15) 水平有关.
之前的发现表明miR-3074-5p增强了EVT亡并减少了入侵.

研究的目的:

阐明miR-3074-5p/GDF15通路在调节EVT功能中的作用.
在EVT中调查miR-3074-5p,EIF2S1和GDF15之间的调节机制.

主要方法:

从RM患者的样中对miR-3074-5p,GDF15和EIF2S1表达的定量分析.
使用HTR8/SVneo细胞进行体外研究,以评估miR-3074-5p和GDF15操纵对EVT增殖,迁移和入侵的影响.
路西法雷斯记者测定证实了miR-3074-5p.直接针对EIF2S1的测定.

主要成果:

在RM患者中,Villus GDF15和EIF2S1表达显著下降.
miR-3074-5p直接针对EIF2S1,导致GDF15生产减少.
抑制GDF15抑制了HTR8/SVneo细胞的增殖,迁移和入侵;因EIF2S1过度表达或GDF15治疗而逆转效应.

结论:

miR-3074-5p通过抑制EIF2S1在EVTs中的表达来降低GDF15的产生.
通过这种途径介导的GDF15功能缺陷可能会通过损害EVT增殖和入侵而导致早期怀孕流产.

关键词:

欧洲投资基金 (EIF2S1)超的热囊细胞.在GDF15中,GDF15是GDF15中的一个.经常发生的流产.在 miR-3074-5p 中使用.

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