卵细胞玻璃化降低其修复精子DNA碎片化的能力,并损害胚胎发育
Niloofar Khajedehi1,2, Rouhollah Fathi3, Vahid Akbarinejad4
1Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Reproductive sciences (Thousand Oaks, Calif.)
|December 27, 2023
概括
卵细胞玻璃化可能会损害修复精子DNA损伤的能力,对胚胎发育产生负面影响. 这种生育保护技术可以破坏关键的DNA修复机制,影响生殖结果.
科学领域:
- 生殖生物学 生殖生物学
- 发育生物学是发展生物学.
- 遗传学 是一个遗传学.
背景情况:
- 卵细胞对于修复精子DNA损伤至关重要,确保下一代的基因组完整性.
- 影响卵细胞DNA修复的因素可能对生育能力和后代健康产生重大影响.
- 卵细胞玻璃化是一种常见的生育保存方法,但它对卵细胞修复能力的影响尚未完全理解.
研究的目的:
- 调查卵细胞玻璃化是否影响卵细胞修复精子DNA损伤的能力.
- 评估玻璃化卵子和受损精子对随后胚胎发育的影响.
- 为了检查关键的DNA修复基因的表达,以应对精子损伤和卵细胞玻璃化.
主要方法:
- 在雄性DBA/2小鼠中使用 tert-Butyl氧化物 (tBHP) 诱导了精子DNA损伤.
- 来自雌性DBA/2小鼠的积卵细胞复合体 (COC) 通过Cryotop方法进行了玻璃化.
- 新鲜和玻璃化卵子被用tBHP处理和未处理的精子受精,并监测胚胎发育.
- 通过实时PCR,分析了DNA修复基因 (Mre11a,Rad51,Brca1,Xrcc4) 的表达在阴茎和胚囊中.
主要成果:
- tBHP治疗显著增加了精子DNA碎片化和降低了精子质量.
- 受精和早期胚胎发育率下降,特别是在使用tBHP处理精子和玻璃化卵子细胞的组中.
- 卵细胞的玻璃化,特别是当与受损的精子相结合时,导致了最低的胚胎细胞发育率.
- DNA修复基因 (Brca1,Rad51,Xrcc4) 的表达在囊和囊胚细胞中发生了改变,这表明修复反应受到干扰.
结论:
- 卵细胞玻璃化可能会损害卵细胞修复精子DNA碎片化的能力.
- 由于玻璃化而导致的DNA修复损伤会对胚胎发育产生负面影响,特别是当精子DNA完整性受到损害时.
- 这些发现突出了与使用玻璃化卵子细胞用于保存生育性相关的潜在风险,当精子质量低于最佳时.
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