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PETR:一种新的围混合管状生物反应器,模拟人类结肠条件

David Vorländer^1,2, Gábor Schultz^1,2, Kristin Hoffmann^1,2

¹Institute of Biochemical Engineering, Technische Universität Braunschweig, Braunschweig, Germany.

Biotechnology and bioengineering

|December 28, 2023

概括

一个新的生物反应器,PEristaltic混合管状生物反应器 (PETR),准确地模仿了人肠道条件,用于体外肠道微生物群的研究. 这种先进的系统可以对肠道健康,益生菌和药物相互作用进行精确,可重复的研究.

科学领域:

生物技术是生物技术.
微生物学微生物学
生物工程是生物工程.

背景情况:

精确的人体结肠体外模型对于了解肠道微生物群至关重要.
现有的模型往往无法复制结肠的动态生理条件.

研究的目的:

介绍和描述一个新的生物反应器,即PEristaltic混合管状生物反应器 (PETR),旨在模拟人类结肠条件.
评估PETR对肠道微生物群的控制体内培养的能力.

主要方法:

该PETR具有模块化,单管设计,定期,以模拟周围静止.
它保持生理温度,厌氧条件和逐渐的pH梯度 (5.57.0).
综合透析系统可以去除短链脂肪酸和水.

主要成果:

在PETR中,平均停留时间为32.8±0.8小时,与成年人结肠相当.
透析系统有效吸收高达60%的短链脂肪酸和850毫升/天的水.
成功种植Bifidobacterium animalis证明了反应器的适用性.

结论:

PETR独特地结合了整个结肠的模拟,周位混合,代谢物吸收和pH梯度在一个系统中.
这种生物反应器为可靠和可再生的微生物群研究提供了重大进步.
潜在的应用包括研究食品化合物,益生菌,益生菌,感染和药物输送.

关键词:

透析是通过透析进行的.在体外模拟模拟肠道模型肠道模型微生物组是一个微生物组.我们的微生物群.在pH梯度的梯度下,

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