菌体内素Ply113作为一种强大的抗菌剂,可以对抗由肠球菌和金黄色葡萄球菌形成的多微生物生物膜
Jing Wang1, Siyu Liang1, Xiaofeng Lu1
1State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Frontiers in microbiology
|December 28, 2023
概括
菌体endolysin Ply113有效地对抗抗生素耐药的细菌,如菌,菌和金黄色葡萄球菌. 这种有前途的抗菌剂对单个和混合细菌生物膜以及体内感染都表现出强烈的活性.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 传染性疾病 传染性疾病
背景情况:
- Enterococcus faecium,Enterococcus faecalis和Staphylococcus aureus中的抗生素耐药性构成一个重大的全球健康威胁.
- 这些细菌经常形成多微生物生物膜,使传统的抗生素治疗无效.
- 迫切需要新的治疗策略来应对这些具有挑战性的感染.
研究的目的:
- 评估菌体endolysin Ply113对关键耐药细菌的抗菌和抗菌膜潜力.
- 评估Ply113在破坏单物种和双物种生物膜中的有效性.
- 为了确定Ply113.3的体内抗菌活性.
主要方法:
- 在体外溶解活性测定对E. faecium,E. faecalis和S. aureus进行检测.
- 传输电子显微镜观察Ply113对细菌细胞结构的影响.
- 对单种和双种生物膜的生物膜抑制测定.
- 在小鼠腹性败血症模型中的体内疗效测试.
主要成果:
- Ply113对E. faecium,E. faecalis和S. aureus,包括耐药菌株,表现出快速和强大的解活性.
- 在广泛的温度 (4-45°C),pH (5.0-8.0) 和NaCl度 (0-400mM) 范围内,内素是稳定的.
- Ply113有效地根除了单种和双种生物膜,以剂量依赖的方式.
- 在体内观察到细菌负载的显著减少.
结论:
- 菌体endolysin Ply113表现出广泛的抗菌和抗菌膜活性.
- Ply113是治疗耐药细菌引起的多微生物感染的有希望的候选者.
- 它的稳定性和有效性在体外和体内突出显示了其作为一种新型抗菌剂的潜力.
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