对比表明,多重聚合酶链反应识别了与感染相关的尿路生物标志物-阳性尿路感染,这些感染被标准尿培养遗漏了
Emery Haley1, Natalie Luke1, Mohit Mathur2
1Department of Clinical Research, Pathnostics, Irvine, CA, USA.
European urology open science
|December 28, 2023
概括
多重聚合酶连锁反应 (M-PCR) 检测出更多的尿路感染 (UTI) 微生物,而不是标准的尿液培养 (SUC). 在M-PCR阳性/SUC阴性病例中感染生物标志物升高表明这些微生物会导致尿路感染.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 传染性疾病 传染性疾病
- 生物标志物研究 生物标志物研究
背景情况:
- 多重聚合酶链反应 (M-PCR) 与标准尿培养 (SUC) 相比,在尿路感染 (UTIs) 中对微生物检测具有更高的灵敏度.
- 这种增加的敏感性引发了关于检测到的微生物是真正的致病原体还是偶然发现的问题.
研究的目的:
- 将症状性尿路感染病例中的感染相关生物标志物水平与M-PCR和SUC.的结果进行比较.
- 为了确定M-PCR检测到的微生物,被SUC遗漏,是否与实际的尿路感染有关.
主要方法:
- 纳入了疑似尿路感染 (n=583) 的症状患者 (≥60岁).
- 用M-PCR和SUC分析尿样以检测微生物.
- 三种感染生物标志物 (中性纤维素凝酶相关的脂卡林,白素-8,白素-1β) 通过ELISA量化.
主要成果:
- 呈M-PCR阳性/SUC阴性病例 (n=80) 的中位数生物标志物水平明显高 (p<0.0001) 于呈M-PCR阴性/SUC阴性病例 (n=107).
- 76%的M-PCR阳性/SUC阴性标本具有两个或两个以上的阳性生物标志物.
- 一个限制是无法将个体生物与炎症联系起来.
结论:
- 在M-PCR阳性/SUC阴性尿路感染病例中,很大一部分患者表现出尿路生物标志物升高,特别是在非大肠杆菌感染中.
- 这些发现强烈表明,M-PCR检测到的,但SUC错过的微生物确实与引起尿路感染有关.
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