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转录组分析显示,垂体lncRNA,circRNA和mRNA影响高和低产山羊的生育能力

Shuaixiang Mao¹, Shucan Dong¹, Biwei Hou¹

¹College of Animal Science, South China Agricultural University, Guangzhou, China.

Frontiers in genetics

|December 28, 2023

概括

研究人员探索了脑下垂体中的非编码RNA (lncRNAs),循环RNA (circRNAs) 和信使RNA (mRNAs) 影响山羊生育的时间. 确定了调节激素分泌和影响莱州山羊生育能力的关键基因.

科学领域:

内分泌学和生殖生物学
分子生物学和基因组学
动物科学动物科学

背景情况:

下垂体是生殖和新陈代谢的中央调节器.
了解垂体功能是改善动物生育能力的关键.
莱州山羊表现出不同程度的生育能力,使他们成为生育研究的典范.

研究的目的:

为了研究 lncRNAs,circRNAs 和mRNAs 在莱州山羊的垂体腺体中的调节作用.
识别特定的非编码RNA和mRNA与山羊生育能力的变化相关.
阐明将 pituitary激素分泌与生殖成功联系起来的分子机制.

主要方法:

来自不同生育水平的莱州山羊的垂体腺样本的转录组分析.
对差异表达RNA的基因本体学 (GO) 和KEGG通路丰富分析.
差异表达的lncRNAs,circRNAs和mRNAs的识别和表征.

主要成果:

观察到特定的垂体lncRNAs (例如,ENSCHIT00000010293) 和circRNAs (例如,chicirc_029285) 的差异表达,与通过宿主基因分泌的激素有关.
几种mRNA (GABBR2,SYCP1,HNF4A,CBLN1,CDKN1A) 被确定为差异表达并影响山羊的生育能力.
丰富分析突出了与激素分泌,生殖细胞发育和GnRH分泌相关的途径.

结论:

pituitary 腺体中的特定 lncRNAs 和 circRNAs 在调节激素分泌,从而促进山羊生育方面发挥作用.
鉴定到的mRNAs通过调节垂体激素分泌直接影响生殖成功.
这些发现为莱州山羊的生育率调节提供了分子洞察力,可能有助于繁殖策略.

关键词:

莱州山羊 - 莱州山羊环RNA 环RNA 是一个环RNA.生育能力生育能力生育能力生育能力在ncRNA中,我们可以它们是mRNARNA.pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary 下垂体下垂体下垂体下垂体下垂体下垂体下垂体下垂体

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