相关实验视频

Updated: Jul 7, 2025

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

Published on: January 13, 2012

在小鼠中,PI3KCIIα-依赖的自程序在应对低剪压时保护从内皮功能障碍和动脉样硬化

Mouin Nasr¹, Alexis Fay¹, Adrien Lupieri¹

¹Institute of Metabolic and Cardiovascular Diseases (I2MC), Institut national de la Santé et de la Recherche (INSERM) 1297, University of Toulouse 3, France (M.N., A.F., A.L., N.M., A.D., R.Z., A.S., A.W., J.V., A.N.-S., D.R., M.L., S.G.).

Arteriosclerosis, thrombosis, and vascular biology

|December 28, 2023

概括

低和高切割应力通过PI3KCIIα差异调节内皮细胞自. 这条涉及PI3KCIIα和mTORC1的途径对于防止低剪切应力区域内内皮细胞功能障碍至关重要.

科学领域:

心血管生物学心血管生物学
细胞机械传导细胞机械传导
自学研究自学研究

背景情况:

内皮细胞 (ECs) 必须对机械力量做出反应,例如低切削应力 (LSS) 和高切削应力 (HSS) 适应恒温.
动脉LSS和HSS对EC自产生不同的影响,但具体机制尚不清楚.

研究的目的:

研究细胞内皮中对LSS和HSS的自反应的独特细胞和分子机制.
阐明特定信号通路在调解这些反应和维持EC功能中的作用.

主要方法:

使用流体流系统将LSS和HSS应用于EC.
对ApoE-/-小鼠的动脉进行了多尺度成像分析,以研究自反应.
研究了氨基酸3-激酶 (PI3Ks) 和mTORC1通路的参与.

主要成果:

通过不同的信号模块,LSS和HSS激活了自.
由LSS诱导的自受酸3-激酶II类α (PI3KCIIα) 的调节,而不是VPS34.
在ApoE-/-小鼠中减少PI3KCIIα导致EC功能障碍和LSS区域的斑块增加,与初级纤毛生物发生障碍有关.

结论:

动脉中的机械力变化决定了EC自反应.
PI3KCIIα/mTORC1轴在防止LSS区域的EC功能障碍方面发挥着至关重要的作用.
内皮的初级毛可能充当机械传感器,将PI3KCIIα与EC稳态联系起来.

关键词:

动脉样硬化动脉样硬化自自是自的过程.细胞内皮细胞的内皮细胞.酸胺3-酶酶的作用是什么主要的纤毛是主要的纤毛.剪切压力的压力.

更多相关视频

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Published on: June 22, 2010

En Face Preparation of Mouse Blood Vessels

En Face Preparation of Mouse Blood Vessels

Published on: May 19, 2017

相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...