简单的途径稳定同位素编码原生甘氨酸
Johannes Helm1, Clemens Grünwald-Gruber1, Jonathan Urteil1
1Department of Chemistry, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria.
Analytical chemistry
|December 28, 2023
概括
一种新方法使用水酸用于稳定同位素标记甘氨酸,使生物研究能够更好地识别和分离同位素. 这种方法提供了一种更安全,更负担得起的方法来创建基本的甘氨酸标准.
科学领域:
- 格莱科米克斯 (Glycomics) 是一个有趣的药物.
- 质谱测量质量谱测量
- 碳水化合物化学 碳水化合物化学
背景情况:
- 准确识别甘氨酸对于理解它们的生物作用至关重要.
- 稳定同位素标记的甘氨酸在基于质谱的甘氨酸分析中,对于保持时间的正常化至关重要.
- 目前生产标记糖的方法涉及化学酶或酶方法,这些方法可能是复杂的或引入特定的标签.
研究的目的:
- 开发一种更容易获得和更安全的方法,用于将稳定同位素引入甘中.
- 为了使大量编码的甘氨酸能够用于先进的分析应用.
- 为生成异构体特定研究的甘氨酸标准提供一个多功能平台.
主要方法:
- 使用水酸盐去化甘氨酸,这是一种比无水水更危险和更实惠的试剂.
- 反应条件 (时间,温度) 的优化,以完成中间产品的转化和分离.
- 将重同位素引入去N-乙化甘氨酸中,以创建质量编码标准.
主要成果:
- 氨酸水合物有效地实现了脱N乙化,使重同位素的纳入成为可能.
- 在100°C的温度下,在72小时内实现了双年性甘氨酸 (含有或不含有酸) 的完全转化.
- 较短的潜伏时间允许隔离具有自由氨基群的定义度的中间体,从而使可变同位素的纳入成为可能.
结论:
- 描述的方法提供了一种通用且具有成本效益的途径,以大规模编码的甘氨酸.
- 这些标记的甘氨酸可以作为各种甘氨酸类型的同位素特异性LC-MS分析的内部标准,包括N-甘氨酸,O-甘氨酸和母乳寡糖.
- 这种方法提高了生物研究中糖甘分析的可靠性和范围.
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