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Xuelin Wang¹, Bei Jia², Kyungsene Lee¹

¹Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Journal of the American Chemical Society

|December 28, 2023

概括

研究人员开发了一种新型的仿生细菌囊,使用酸盐聚氨酸涂层在活细胞上显示体. 这种方法增强了细胞的识别和稳定性,提高了自然杀手细胞的向效率.

科学领域:

生物材料科学
细胞生物学
免疫技术

背景情况:

哺乳动物细胞封装对于糖尿病治疗和3D打印等应用至关重要.
现有的方法缺乏功能分子的高效表面显示,例如用于增强细胞识别.
需要一种生物模拟方法,以在细胞表面上显示稳定的aptamer.

研究的目的:

合成一种新的仿生细菌囊,
通过使用自然杀手 (NK) 细胞作为模型系统,增强细胞识别能力.
通过新型涂层方法评估阿普坦显示的稳定性和有效性.

主要方法:

合成了一种超薄的阿尔吉纳特聚氨酸涂层用于活细胞表面功能.
在自然杀手 (NK) 细胞表面显示高亲和度的体.
与胆固醇插入相比,评估的阿普塔默显示稳定性.
对被涂层的NK细胞对目标细胞的识别和杀死效率进行了评估.

主要成果:

酸盐聚氨酸涂层成功地显示了NK细胞表面上的体.
细胞表面的阿帕特马半衰期从< 1.5 小时显著增加到> 20 小时.
涂层NK细胞在识别和消除细胞方面表现出更高的效率.
通过涂层介导的aptamer显示显示比直接的脂质双层插入更稳定.

结论:

开发了一种新的稳定的仿生细菌囊涂层,用于在活细胞上显示aptamer.
这种方法显著提高了细胞表面结合的体的稳定性和有效性.
这项技术有望改善细胞识别和治疗向,特别是基于NK细胞的免疫疗法.

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