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通过加强前体供应和产品分泌,增强menaquinone-7生物合成

Pengchen Hu¹, Cheng Peng¹, Bei Zhang¹

¹College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.

Bioprocess and biosystems engineering

|December 28, 2023

概括

在Bacillus natto中增强的前体供应显著增加了menaquinone-7 (MK-7) 产量. 这项研究优化了前体组合和食策略,以提高MK-7的产量,从而获得潜在的健康益处.

科学领域:

生物技术是生物技术.
微生物发酵微生物发酵
生物化学生物化学

背景情况:

menaquinone-7 (MK-7) 是维生素K2的一个重要形式,在人类健康中起着至关重要的作用,特别是在预防骨质疏松症和心血管疾病方面.
目前MK-7的合成方法由于复杂的代谢途径,效率较低.
优化前体的可用性是提高MK-7生物合成的关键.

研究的目的:

研究增强前体供应对Bacillus natto.中MK-7合成的影响.
确定最佳的前体度,组合和食时间,以最大限度地提高MK-7的产量.
开发一种新的发酵策略,以提高MK-7的产量和生产率.

主要方法:

对三个关键前体的系统研究:酸,石基胺酸和谷氨酸.
在发酵过程中优化前体度,组合和添加时间.
与MK-7生物合成相关的基因表达的分析.
开发和实施一种结合前体增强和产品分泌策略.

主要成果:

希基米酸和谷氨酸的组合使MK-7的产量翻了一番,达到50mg/L,生产率为0.52mg/L·h.
最初添加了石基胺酸和谷氨酸,随后添加了pyruvate,进一步增加了MK-7的产量,达到58 mg/L.
与MK-7合成相关的基因表达显著上调.
新的发酵策略实现了MK-7产量为63 mg/L,生产率为0.45 mg/L·h.

结论:

增强前体供应,特别是石基米酸和谷氨酸,是促进Bacillus nattoMK-7生物合成的有效策略.
优化的发酵条件和养策略显著提高了MK-7的标位和生产力.
这项研究提出了一种新的发酵调节策略,以促进维生素K2的生产.

关键词:

纳托杆菌 (Bacillus natto) 是一种植物.发酵发酵是一个过程.梅纳金-7是什么意思它们的前身是前体.维生素K2 维生素K2 是一种维生素.

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