伴侣蛋白Cct5对于血液形成干细胞的维护至关重要
Linxi Li1, Jie Huang2, Suping Zhang1
1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, 280, Chong-Qing South Road, West Bldg #2, Rm 804, Shanghai, 200025, China.
Stem cell reviews and reports
|December 28, 2023
概括
对于蛋白质折叠至关重要的TRiC/CCT复合体,对于维持造血干细胞和祖先细胞 (HSPCs) 至关重要. 失去Cct5会损害HSPC的功能及其产生髓状和淋巴状细胞的能力.
科学领域:
- 细胞生物学 细胞生物学
- 血液形成 血液形成 血液形成
- 分子生物学分子生物学
背景情况:
- 蛋白质稳定对于造血干细胞和原生细胞 (HSPCs) 的长期维持至关重要.
- 在HSPC中TRiC/CCT chaperonin复合物的特定作用仍然在很大程度上是未知的.
研究的目的:
- 研究TRiC/CCT复合物的功能,特别是Cct5子单元,在小鼠HSPC的维护和功能.
- 为了确定Cct5在HSPCs中调节恒温的分子相互作用.
主要方法:
- 在小鼠HSPC中Cct5的条件淘汰 (KO).
- 主要和次要竞争性移植测定.
- 整合蛋白质-蛋白质相互作用 (PPI) 数据库与RNA测序 (RNA-seq) 数据.
主要成果:
- 条件Cct5KO显著影响HSPC的维护.
- Cct5 KO HSPCs无法在移植后重建髓状和淋巴状血统.
- Cct5与关键的转录因子 (MYC,PIAS1,TP53,ESR1,HOXA1,JUN) 相互作用,调节自,骨髓分化和细胞骨组织.
结论:
- 通过Cct5,TRiC/CCT复合体在HSPC的维护和功能中发挥着至关重要的作用.
- 通过cct5介导的蛋白质稳定对于调节控制高细胞平衡的转录网络至关重要.
- 干扰Cct5影响HSPCs的髓状和淋巴状血统的发展.
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