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在水定压力和偏离性菌株下,在牛年纤维细胞中细胞行为和细胞外矩阵周转
Ryo Taiji1, James D Kang1, Shuichi Mizuno1
1Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
概括
脊柱负荷会影响纤维环 (AF) 细胞,减少矩阵合成和增殖. 避免持续的超载对于促进AF组织在受伤后的修复和再生至关重要.
科学领域:
- 生物医学工程 生物医学工程
- 整形外科 整形外科 整形外科
- 再生医学是一种再生医学.
背景情况:
- 椎间盘是常见的脊椎疾病,通常需要切除椎间盘.
- 了解状纤维 (AF) 细胞对脊柱负荷的反应,是开发AF撕裂治疗策略的关键.
- 以前的研究表明,脊柱负荷会影响AF细胞功能,但内外AF细胞的特定反应需要进一步阐明.
研究的目的:
- 为了研究模拟脊柱负荷对牛内外环纤维细胞 (AF) 的合成和降解活动的影响.
- 为了评估水静压 (HP) 和弧应变 (Strain) 对AF细胞增殖和细胞外基因表达的影响.
- 为了确定可能促进AF组织再生的最佳负载条件.
主要方法:
- 牛的AF细胞 (内部和外部) 受到水静压 (HP),弧应变 (Strain) 和组合的HP/Strain,模仿脊柱负荷.
- 使用RT-PCR评估了关键矩阵分子 (例如,aggrecan,原类型) 和细胞增殖标记物 (增殖细胞核抗原) 的基因表达.
- 免疫组织化学被用来验证基因表达的发现.
主要成果:
- 在HP下,内部AF细胞显示了metalloproteinases-2的增加的aggrecan和组织抑制剂.
- 外部AF细胞在Strain和HP/Strain下表现出降低的原蛋白类型-2,原蛋白类型-1和弹性质表达.
- 细胞增殖 (增殖细胞核抗原) 在 Strain/HP/Strain 条件下在内部AF细胞中增加,但在无负荷和HP条件下在外部AF细胞中减少.
结论:
- 模拟的脊柱负荷对内外AF细胞产生不同影响,影响矩阵合成和细胞增殖.
- 综合的水静压和应变似乎减少了合成和降解活动,同时增加了内部AF细胞的增殖.
- 应避免连续的过载,以防止减少合成活动和纤维状环中有限的组织修复.
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