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通过数字全息显微镜对 diatom frustules 的蚀刻进行成像和定量分析

Maria Christine Lugo¹, Makoto Saito¹, Masaki Kitamura¹

¹Department of Physics, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan.

ACS biomaterials science & engineering

|December 28, 2023

概括

数字全息显微镜 (DHM) 提供了一种新的方法来测量藻. 这种方法可以实时观察果的降解,并提供有关其结构和特性的见解.

科学领域:

生物材料科学生物材料科学
显微镜技术显微镜技术
二原子生物学二原子生物学

背景情况:

原子体具有复杂的多孔结构,使它们成为物理性质修改的目标.
由于其复杂的架构和现有方法的局限性,精确的三维 (3D) 测量变化是很困难的.

研究的目的:

引入一种使用数字全息显微镜 (DHM) 分析蚀刻藻的新方法.
为了实现实时的,单细胞水平的观察果的降解过程.

主要方法:

数字全息显微镜 (DHM) 用于分析来自Craspedostauros sp.的果. 藻. 这是一种藻.
在25°C和60°C下使用1N NaOH在5分钟内蚀刻Frostule.
DHM结合全息和断层扫描来重建3D折射率图像以进行维度分析.

主要成果:

这项研究成功地测量了的宽度,体积和表面积.
对于在60°C时刻刻的果与未刻刻的对照相比,观察到显著的测量差异,尽管具有很高的可变性.
通过实时观察缩的NaOH中果的降解,获得了实时观察.

结论:

这项研究展示了第一次实时,单细胞水平观察藻的蚀刻.
DHM提供了一种可访问的方法,用于3D测量,可能产生新的基本见解和应用.

关键词:

数字全息显微镜的数字全息显微镜.蚀刻蚀刻蚀刻是一种方法.在单细胞水平上.

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