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Updated: Jul 6, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

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设计一个混合链反应探测器用于多重转录检测,使用高水平成像.

Dongjian Cao1, Xinxin Qin1, Wenjing Wang1

  • 1Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

ACS nano
|December 28, 2023
PubMed
概括

这项研究引入了改进的混合链反应 (HCR) 探针,用于在多重 FISH 试验中增强信号放大. 新的ISRCA-HCR方法显著提高了空间细胞类型和转录组分析的成像质量.

关键词:
细胞类型 细胞类型光在现场杂交 (FISH) 的方法混合连锁反应 (HCR) 是一种混合连锁反应.滚动圈放大器 (RCA) 是一种空间转录组空间转录组

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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