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在bEECs中LPS诱导的上皮层介质细胞过渡的机制

Mingkun Sun¹, Pengjie Song¹, Yu Zhao¹

¹College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, China.

|December 28, 2023

概括

高度饮食增加了奶牛的脂多糖 (LPS),通过诱导表皮-介质细胞过渡 (EMT) 来破坏胚胎植入. Bta-miR-200b通过准ZEB1来抵消这种影响,为改善奶牛繁殖提供了洞察力.

科学领域:

生殖生物学生殖生物学
细胞和分子生物学是细胞和分子生物学.
动物科学动物科学

背景情况:

乳牛的高度饮食可能导致亚急性乳头酸和血脂多糖化物 (LPS) 水平升高.
牛的LPS峰值水平与胚胎植入的关键时期相吻合,这表明可能与生殖问题有关.

研究的目的:

调查高水平的LPS是否会在牛子宫内皮细胞 (bEECs) 中诱导异常的上皮-介质细胞过渡 (EMT).
探索ZEB1和Bta-miR-200b在LPS诱导的EMT中的作用及其对母牛胚胎植入的影响.

主要方法:

用LPS对bEEC进行处理,以分析EMT标记物的蛋白质和基因表达 (ZEB1,E-cadherin,N-cadherin,Vimentin).
在暴露于LPS后评估细胞形态和迁移能力.
在ZEB1上研究Bta-miR-200b的调控作用,使用双露西法酶记者测定和模仿转染.

主要成果:

在bEECs中,LPS治疗增加了ZEB1,N-cadherin和Vimentin的表达,同时减少了E-cadherin的表达.
暴露于LPS增强了bEECs的迁徙能力,并诱导了间酶体表型.
发现Bta-miR-200b针对ZEB1的3'UTR,其上调可以逆转LPS诱导的EMT标记.

结论:

通过对ZEB1进行上调,LPS在bEEC中诱导EMT,这有助于在养高缩食的奶牛中导致胚胎植入受损.
Bta-miR-200b通过向ZEB1来抑制LPS诱导的EMT,从而成为改善奶牛生殖率的潜在治疗标.

关键词:

在 Bta-miR-200bb 中.牛牛牛牛牛牛牛子宫内膜上皮细胞是子宫内膜上皮细胞.表皮介质介质过渡的表皮介质.在LPS中使用LPS.

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