在微柱阵列列列中带宽的理论计算
Ali Moussa1, Bram Huygens1, Claudia Venditti2
1Department of Chemical Engineering, Vrije Universiteit Brussel, Brussels, Belgium.
Journal of chromatography. A
|December 28, 2023
概括
我们开发了一个理论模型,用于带宽在微柱阵列列和有多孔涂层的微柱阵列列中. 这个模型揭示了多孔层中的垂直扩散如何抑制带宽,显示出对保留因子的异常依赖.
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 微柱阵列列柱在染色学中被广泛使用.
- 带宽是影响色谱分离效率的关键因素.
- 在柱面上涂覆的多孔材料可以影响带宽.
研究的目的:
- 建立一个理论的板高表达式,用于带宽在微柱阵列列和多孔涂层的微柱阵列.
- 为了研究垂直扩散传输在多孔层对带宽的影响.
- 分析带宽扩大对保留因子的异常依赖.
主要方法:
- 在3D柱阵列中,带宽的理论建模.
- 假设在多孔层中存在同otropic 扩散.
- 移动区域质量转移阻力项 (hCm) 的分析.
主要成果:
- 在多孔外层中的垂直扩散传输显著抑制了带宽.
- 观察到波段扩大对保留因子 (k'') 的异常依赖,偏离了经典的泰勒-阿里斯行为.
- 偏差随着柱子的高度和速度的降低而增加,并受到保持平衡常数 (K) 的影响.
结论:
- 由于复杂的k''-依赖性,很难建立一个一般的板高方程.
- 开发了一种板高表达式,准确度高达k'=4并且对于特定的柱高与柱间距离的比率.
- 分析模型在有限的外部孔隙范围 (ε=0.5) 内是有效的.
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