使用3D打印传感器在人体血中检测阿波莫尔芬的电位测量检测
Manar M Elhassan1, Dalton L Glasco2, Anjaiah Sheelam2
1Department of Chemistry, Washington State University, Pullman, WA, 99163, USA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El-Sherouk City, 11837, Egypt.
一种新的3D打印电位计传感器提供了一种低成本的选择性方法,用于检测帕金森病的关键药物阿波莫芬,无论是散装样本还是人体血样本. 这一进步有助于管理帕金森病,因为它可以精确监测阿波莫尔芬水平.
科学领域:
- 分析化学 分析化学
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
背景情况:
- 阿波莫芬是帕金森病管理的关键多巴胺激动剂,主要用于减少"休息时间"症状复发.
- 在生物样本中精确量化阿波莫尔芬对于治疗药物监测和治疗优化至关重要.
- 现有的阿波莫尔芬测定方法可能很复杂或昂贵,需要更简单,更容易获得的分析工具.
研究的目的:
- 设计和制造第一个潜在度传感器用于阿波摩尔芬的确定.
- 开发一种使用立体立体3D打印的快速,低成本的制造方法.
- 为了验证传感器在批量样本和复杂矩阵 (如人体等离子体) 中的性能.
主要方法:
- 使用立体立体3D打印制造固体接触型阿波摩芬离子选择性电极.
- 整合碳网/热塑复合物作为离子对电子传感器.
- 整合了3D打印的离子选择性膜,并添加了calix[6]arene离子体.
主要成果:
- 传感器在生物学相关的度范围 (5.0 mM9.8 μM) 中表现出线性,Nernstian反应 (58.8 mV/十年).
- 对常见的生物离子 (Na +,K +,Mg2 +,Ca2 +) 和利沃多巴 (L-Dopa) 证明了高选择性.
- 传感器表现出良好的稳定性,保质期近4周,在白蛋白的存在下选择性检测自由阿波莫尔芬.
结论:
- 立体立体3D打印可快速且经济高效地制造出一种新型的阿波莫芬电位传感器.
- 开发的传感器可以选择性和灵敏地检测散装和尖的人类血样本中的阿波莫尔芬.
- 这种3D打印的传感器代表了对帕金森病患者中阿波莫尔芬临床监测的有希望的工具.
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