在废水中分析病原性原生动物及其功能途径,使用18S rRNA和猎枪甲基因组学
Nonsikelelo P Mthethwa-Hlongwa1, Isaac D Amoah2, Andres Gomez3
1Institute for Water and Wastewater Technology, Durban University of Technology, Durban 4000, South Africa; Department Community Health Studies, Faculty of Health Sciences, Durban University of Technology, Durban 4000, South Africa.
The Science of the total environment
|December 28, 2023
概括
南非的污水处理厂有各种原生动物,包括像Cryptosporidium和Entamoeba这样的人类病原体. 这些发现凸显了不充分消毒废水对公共健康的潜在风险.
科学领域:
- 环境微生物学环境微生物学
- 寄生虫学的寄生虫学
- 公共卫生 公共卫生
背景情况:
- 由于度低且复杂多样性的原因,环境样本中的真核细胞原生体成分仍然不太清楚.
- 缺乏参考数据库和标准化协议,阻碍了对寄生虫群体的准确分析.
研究的目的:
- 在南非废水中描述原生动物群落和功能途径,使用18S rRNA和猎枪元基因组学.
- 评估原生动物在经过处理和未经处理的废水中存在的公共卫生影响.
主要方法:
- 18S rRNA基因扩增序列测序用于分析原生动物的多样性.
- 枪支元基因组测序为功能途径提供了洞察力,并证实了特定病原体的存在.
- 从南非各个地区的经过处理和未经处理的污水源收集废水样本.
主要成果:
- 原生动物的多样性和组成受到废水处理状态和地理位置的重大影响.
- 未经处理的废水显示了自由生活,寄生虫和潜在的致病原体的高流行率,包括Alveolata,Excavata和Amoebozoa.
- 确定了关键的人类病原体,如Cryptosporidium,Entamoeba histolytica,Blastocystis hominis和Giardia intestinalis,以及毒性基因和丰富的代谢途径.
结论:
- 污水处理厂可以容纳原生动物病原体,具有生长和传播的潜力.
- 废水不充分消毒对人类和动物健康构成重大风险.
- 这项研究为未来关于公众健康问题的废水相关原生动物群落的研究奠定了关键的基础.
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