相关实验视频

Updated: Aug 4, 2026

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Published on: August 25, 2019

微素-LR和微素-RR对斑马鱼幼虫生长和发育的联合抑制作用

Xiao-Chun Guo¹, Shao-Yong Lu¹, Sheng-Nan Zhang²

¹National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, State Environmental Protection Key Laboratory for Lake Pollution Control, State Environmental Protection Scientific Observation and Research Station for Lake Dongtinghu (SEPSORSLD), State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

|December 28, 2023

概括

微囊 (MCs),包括MCLR和MCRR,在斑马鱼中显示出协同的发育毒性. 对这些毒素的同时暴露会破坏生长,甲状腺激素,并导致氧化应激.

科学领域:

环境毒理学环境毒理学
发育生物学是发展生物学.
水生物质毒理学研究

背景情况:

微囊 (MCs) 是一种流行且有毒的蓝藻细菌毒素.
微cystin-leucine-arginine (MCLR) 和微cystin-arginine-arginine (MCRR) 是常见的MC变体. 微cystin-leucine-arginine (MCLR) 和微cystin-arginine-arginine (MCRR) 是常见的MC变体. 微cystin-leucine-arginine (MCLR) 和微cystin-arginine-arginine (MCRR) 是常见的MC变体. 微cystin-leucine-arginine (MCLR) 和微cystin-arginine-arginine (MCRR) 是常见的MC变体. 微cystin-leucine-arginine (MCLR) 和微cystin-arginine-arginine (MCRR) 是常见的MC变体.
在发育过程中MC之间的潜在相互作用是不太了解.

研究的目的:

在早期斑马鱼中研究MCLR和MCRR的联合发育毒性.
评估同时暴露对甲状腺激素水平和基因表达的影响.
为了评估氧化应激和脂质过氧化标志物.

主要方法:

斑马鱼胚胎 (受精后2120小时) 暴露于MCLR和MCRR.
测量了甲状腺激素水平 (T3,T4).
分析了与生长和发育相关的基因表达.
评估了氧化应激的生物标志物 (超氧化脱酶,谷氨,甲,催化酶).

主要成果:

单独的MCRR仅略微增加了T3和T4;单独的MCLR显著增加了它们,这表明有合作效应.
同时暴露导致生长抑制和改变与生长相关基因的表达.
联合暴露诱导了氧化应激和脂质过氧化,由改变的抗氧化酶和MDA水平表明.

结论:

在斑马鱼中,MCLR和MCRR表现出协同的发育毒性.
同时暴露会影响甲状腺激素调节,生长,并诱导显著的氧化应激.
这项研究强调了水生环境中微囊同时存在的风险.

关键词:

发展性毒性发展性毒性脂质过氧化是一种过氧化.在MC-LR中,它是最重要的.在MC-RRRR中.氧化应激是一种氧化应激.斑马鱼胚胎斑马鱼的胚胎

更多相关视频

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Published on: August 23, 2022

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...