整合基因工程和多因素发酵优化,以在Schizochytrium sp中共同生产胡卜素和DHA
Zi-Xu Zhang1, Lu-Wei Xu1, Ying-Shuang Xu1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Xuelin Road, Qixia District, Nanjing, China.
Bioresource technology
|December 28, 2023
概括
这项研究增强了Schizochytrium sp.中的胡卜素和多可萨赫萨酸 (DHA) 生产. 使用优化的发酵和经济高效的玉米炉水解剂,实现创纪录的产量.
科学领域:
- 生物技术是生物技术.
- 微藻的生产 微藻的生产
- 代谢工程是代谢工程.
背景情况:
- 精神分裂 (Schizochytrium sp.) 是一种疾病. 是一种富含脂质的微藻,能够共同生产多可胺酸 (DHA) 和胡卜素.
- 探索和增强Schizochytrium sp.中的天然胡卜素生产. 对于其生物技术应用至关重要.
研究的目的:
- 系统地研究和优化在Schizochytrium sp.中的胡卜素和DHA的联合生产.
- 为了实现β-胡卜素标位的显著增加,同时保持高DHA含量.
- 评估使用玉米炉水解剂用于增强微藻种植的成本效益.
主要方法:
- 增强杰拉尼尔基拉尼尔二酸盐前体的供应.
- 通过糖限发酵来调节碳源.
- 使用响应表面方法和人工神经网络优化源.
- 在5L生物反应器中进行培养.
主要成果:
- 贝塔胡卜素标位增加了43倍,达到创纪录的653.2 mg/L.
- 高水平的多可萨赫萨酸 (DHA) 含量保持在13.4g/L.
- 使用玉米炉水解剂的结果是,总胡卜素和DHA标位分别为502.0 mg/L和13.2 g/L,同时降低了生产成本.
结论:
- 在Schizochytrium sp.中共同生产胡卜素和DHA的高效和经济有效的方法. 已经开发出来了.
- 优化的发酵策略显著提高了β-胡卜素的生产,而不会影响DHA产量.
- 玉米炉水解剂为工业规模的微藻联合生产提供了可行的和经济的基质.
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