猪H3K4me3,H3K27ac和基因表达特征显示了可移植元素的生殖组织特异性活性
Tao Jiang1, Zhi-Min Zhou1, Zi-Qi Ling1
1National Key Laboratory of Pig Genetic Improvement and Germplasm Innovation, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Zoological research
|December 29, 2023
概括
可转移元素 (TE) 在基因调节和组织特异性表达中起着至关重要的作用. 这项研究揭示了TE嵌入基因组,影响基因功能,特别是在生殖组织中.
科学领域:
- 基因组学和分子生物学
- 表观遗传学和基因调控
- 生物研究中的动物模型
背景情况:
- 调节序列和可转移元素 (TE) 构成了基因组的重要部分,但它们在基因转录中的功能,特别是组织特异性表达,尚不清楚.
- 猪是基因组研究的有价值模型,因为它们有多样化的野生和养种群.
研究的目的:
- 通过使用综合基因组数据,对猪的调控元素和试验结果进行注释.
- 研究TEs和调控元素与各种组织和发育阶段的基因修饰和基因表达的关联.
- 阐明TE在组织特异性基因调节中的功能性作用.
主要方法:
- 综合分析H3K27ac ChIP-seq,H3K4me3 ChIP-seq和RNA-seq数据来自10个胎儿和成年猪的组织.
- 相关性分析,以评估mRNA表达和基因素修饰峰值活动之间的关系.
- 与监管区域重叠的可转移元素的识别和描述.
主要成果:
- H3K27ac与基因表达的相关性比H3K4me3.3更强.
- 1.45%的TEs与H3K27ac或H3K4me3峰值重叠,其中大多数具有组织特异性活性.
- 在卵巢H3K27ac峰值中丰富了一种特定的TE亚家族 (LTR4C_SS),含有SIX1/SIX4结合基因.
- 4,688个含有TE的转录显示出不同的组织和特定阶段的表达,其中1,967个在丸中进行了丰富.
- 在SRPK2基因中鉴定出一种丸特异性的替代性促进物 (LTR MLT1F1),在人类和小鼠中保存.
结论:
- 可移植的元素是基因组的组成部分,具有重要的组织特异性功能,特别是在生殖器官.
- 转基因可以充当调节元素,影响不同组织和发育阶段的基因表达模式.
- 这些发现突显了TE在塑造基因调节和潜在地对物种特异性特征作出贡献方面的进化重要性.
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