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Updated: Jul 6, 2025

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一种阿斯巴拉基尼尔内酶的细胞内应用,用于产生重组头到尾循环蛋白

T M Simon Tang1, Jody M Mason1

  • 1Department of Life Sciences, University of Bath, Claverton Down, Bath, North Somerset BA2 7AY, U.K.

JACS Au
|December 29, 2023
PubMed
概括

研究人员开发了一种新的方法,利用植物酶在细菌中制造循环蛋白. 这种可持续的方法提高了蛋白质的稳定性和活性,为各种应用提供了一种简化替代化学合成的方法.

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Tail-anchoring of Proteins in the ER Membrane01:45

Tail-anchoring of Proteins in the ER Membrane

Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
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